International Journal of Nanoscience and Nanotechnology

International Journal of Nanoscience and Nanotechnology

Laser-Assisted Synthesis and Modification of Nanodiamond/Gold Nanocomposites: A Novel Therapeutic Approach for Burn Wound Healing in Diabetic Rats

Document Type : Research Paper

Authors
fariba mehradnia 1
BATOOL SAJAD 1
Farideh Bahrami 2
Seyedeh Soraya Mousavi 3
Seyyed Ahad Bagheri 2
Seyyed Homayoun Sadraei 4
Javad Raouf Sarshoori 5
1 Department of Atomic and Molecular Physics, Faculty of Physics, Alzahra University, Tehran, Iran
2 Department of Physiology and Medical Physics, Faculty of Medicine, Baqyatoolah University of Medical Sciences, Tehran, Iran
3 tehran
4 Department of Anatomy, Faculty of Medicine, Baqyatoolah University of Medical Sciences, Tehran, Iran
5 Department of AnatomyŁ«Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran,Iran.
10.22034/ijnn.2026.2050371.2624
Abstract
This study presents a novel laser-assisted method for synthesizing a nanocomposite of nanodiamonds (NDs) and gold nanoparticles (Au NPs). Our approach uniquely uses laser treatment to simultaneously create the nanocomposite and modify the ND structure, enhancing its biomedical properties.
We evaluated the therapeutic potential of this nanocomposite in diabetic wound healing studies using highly diabetic rat models, where it demonstrated superior efficacy compared to other treatments. Individual treatments with NDs and Au NPs significantly improved over the control group. higher wound closure rates by Day 7 compared to ND, AuNP, and control groups (p < 0.001). Re-epithelialization analysis on Day 21 confirmed superior regeneration in the ND/AuNP group versus all other groups (p < 0.001).
These results highlight the promise of our innovative synthesis strategy for advancing therapeutic applications in diabetic wound healing.
Keywords
Nanodiamonds
Gold Nanoparticles
Laser-Assisted Synthesis
Diabetic Wound Healing
Raman Spectroscopy
Nanocomposites
Subjects
1. Bokobza L, Bruneel JL, Couzi M. Raman spectra of carbon-based materials (from graphite to carbon black) and of some silicone composites. C. 2015;1(1):77-94. https://doi.org/10.3390/c1010077
2. Zhang H, Chen X, Yin ZQ. Quantum information processing and precision measurement using a levitated nanodiamond. Adv Quantum Technol. 2021;4(8):2000154. https://doi.org/10.1002/qute.202000154 
3. Montes-García V, Squillaci MA, Diez-Castellnou M, Ong QK, Stellacci F, Samori P. Chemical sensing with Au and Ag nanoparticles. Chem Soc Rev. 2021;50(2):1269-1304. https://doi.org/10.1039/D0CS01112F
4. Torrisi L, Restuccia N. Laser-generated Au nanoparticles for biomedical applications. IRBM. 2018;39(5):307-312. https://doi.org/10.1016/j.irbm.2018.09.005
5. Correard F, Maximova K, Esteve MA, Villard C, Roy M, Al-Kattan A, et al. Gold nanoparticles prepared by laser ablation in aqueous biocompatible solutions: assessment of safety and biological identity for nanomedicine applications. Int J Nanomedicine. 2014;9:54155430. https://doi.org/10.2147/IJN.S65817
6. Havel J, Peña-Méndez EM, Amato F, Panyala NR, Buršíková V. Laser ablation synthesis of new gold carbides: From gold-diamond nanocomposite as a precursor to gold-doped diamonds. Rapid Commun Mass Spectrom. 2014;28(3):297-304. https://doi.org/10.1002/rcm.6783
7. Xiao J, Ouyang G, Liu P, Wang CX, Yang GW. Reversible nanodiamondcarbon onion phase transformations. Nano Lett. 2014;14(6):36453652. https://doi.org/10.1021/nl5014234
8. Amendola V, Meneghetti M. Laser ablation synthesis in solution and size manipulation of noble metal nanoparticles. Phys Chem Chem Phys. 2009;11(20):3805-3821. https://doi.org/10.1039/b900654k
9. Kautek W, Krüger J, Lenzner M. Laser-assisted synthesis of nanomaterials: Fundamentals and applications. Adv Opt Technol. 2018;7(1):17-29.
10. Dudhane AA, Waghmode SR, Dama LB, Mhaindarkar VP, Sonawane A, Katariya S. Synthesis and characterization of gold nanoparticles using plant extract of Terminalia arjuna with antibacterial activity. Int J Nanosci Nanotechnol. 2019;15(2):75-82.
11. Dinç B. Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis elegans. Results Chem. 2025;14:102092. https://doi.org/10.1016/j.rechem.2025.102092 
12. Kumar CSSR, Weissleder R. Regulatory challenges for nanomaterials in medicine: Insights and perspectives. Nat Nanotechnol. 2021;16(8):800-811.
13. Habib A, Tabata M, Wu YG. Formation of gold nanoparticles by Good’s buffers. Bull Chem Soc Jpn. 2005;78(2):262-269. https://doi.org/10.1246/bcsj.78.262 
14. Sylvestre JP, Poulin S, Kabashin AV, Sacher E, Meunier M, Luong JH. Surface chemistry of gold nanoparticles produced by laser ablation in aqueous media. J Phys Chem B. 2004;108(43):16864-16869. https://doi.org/10.1021/jp047134+ 
15. Mezni A, Dammak T, Fkiri A, Mlayah A, Abid Y, Smiri LS. Photochemistry at the surface of gold nanoprisms from surface-enhanced Raman scattering blinking. J Phys Chem C. 2014;118(31):17956-17967. https://doi.org/10.1021/jp5038188
16. Zaitsev AM. Optical Properties of Diamond: A Data Handbook. Springer Science & Business Media; 2013.
17. Guselnikova O, Svorcik V, Lyutakov O, Chehimi MM, Postnikov PS. Preparation of selective and reproducible SERS sensors of Hg2+ ions via a sunlight-induced thiol-yne reaction on gold gratings. Sensors (Basel). 2019;19(9):2110. https://doi.org/10.3390/s19092110
'18. Li H, Kopiec G, Müller F, Nyßen F, Shimizu K, Ceccato M, et al. Spectroscopic evidence for a covalent sigma Au-C bond on Au surfaces using 13C isotope labeling. JACS Au. 2021;1(3):362-368. https://doi.org/10.1021/jacsau.0c00108
19. He XN, Gao Y, Mahjouri-Samani M, Black PN, Allen J, Mitchell M, et al. Surface-enhanced Raman spectroscopy using gold-coated horizontally aligned carbon nanotubes. Nanotechnology. 2012;23(20):205702. https://doi.org/10.1088/0957-4484/23/20/205702
20. Ma Y, Huang Z, Li S, Zhao C. Surface-enhanced Raman spectroscopy on self-assembled Au nanoparticles arrays for pesticides residues multiplex detection under complex environment. Nanomaterials (Basel). 2019;9(3):426. https://doi.org/10.3390/nano9030426
21. Hong S, Li X. Optimal size of gold nanoparticles for surface-enhanced Raman spectroscopy under different conditions. J Nanomater. 2013;2013:790323. https://doi.org/10.1155/2013/790323
22. Shalaby MA, Anwar MM, Saeed H. Nanomaterials for application in wound healing: current state-of-the-art and future perspectives. J Polym Res. 2022;29:91. https://doi.org/10.1007/s10965-021-02870-x
23. Sheikh-Oleslami S, Tao B, D’Souza J, Butt F, Suntharalingam H, Rempel L, et al. A Review of Metal Nanoparticles Embedded in Hydrogel Scaffolds for Wound Healing In Vivo. Gels. 2023;9(7):591. https://doi.org/10.3390/gels9070591
24. Khalid A, Bai D, Abraham AN, Jadhav A, Linklater D, Matusica A, et al. Electrospun nanodiamond-silk fibroin membranes: a multifunctional platform for biosensing and wound-healing applications. ACS Appl Mater Interfaces. 2020;12(43):48408-48419. https://doi.org/10.1021/acsami.0c15612 
25. Wei SC, Chang L, Huang CC, Chang HT. Dual-functional gold nanoparticles with antimicrobial and proangiogenic activities improve the healing of multidrug-resistant bacteria-infected wounds in diabetic mice. Biomater Sci. 2019;7(11):4482-4490. https://doi.org/10.1039/C9BM00772E
26. Shayo GM, Elimbinzi E, Shao GN. Preparation methods, applications, toxicity and mechanisms of silver nanoparticles as a bactericidal agent and superiority of green synthesis method. Heliyon. 2024;10(17):e36539.  https://doi.org/10.1016/j.heliyon.2024.e36539
27. Pino P, Bosco F, Mollea C, Onida B. Antimicrobial Nano-Zinc Oxide Biocomposites for Wound Healing Applications: A Review. Pharmaceutics. 2023;15(3):970. https://doi.org/10.3390/pharmaceutics15030970
28. Singh S. Zinc oxide nanoparticles impacts: cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity. Toxicol Mech Methods. 2019;29(4):300-311. https://doi.org/10.1080/15376516.2018.1553221 
29. Kim SB, Na-Kyung Y, Soo-Jin C. Interactions between ZnO Nanoparticles and Polyphenols Affect Biological Responses. Nanomaterials (Basel). 2022;12(19):3337. https://doi.org/10.3390/nano12193337 
30. Salesa B, Ferrús-Manzano P, Tuñón-Molina A, Cano-Vicent A, Assis M, Andrés J, et al. Study of biological properties of gold nanoparticles: Low toxicity, no proliferative activity, no ability to induce cell gene expression and no antiviral activity. Chem Biol Interact. 2023;382:110646. https://doi.org/10.1016/j.cbi.2023.110646
31. Gupta C, Prakash D, Gupta S. Cancer treatment with nanodiamonds. Front Biosci (Schol Ed). 2017;9(1):62-70. https://doi.org/10.2741/s473 
32. Gunter RM, Sari LK, Hill C. Scalable synthesis of functionalized nanoparticles: Challenges and perspectives. J Nanopart Res. 2023;25(2):45-65.
33. Zhang Y, Sun H, Liu Z. Toxicological profiles of nanomaterials: A comparative analysis. Nano Today. 2022;40:101191.
International Journal of Nanoscience and Nanotechnology
Volume 21, Issue 2
Spring 2025
Pages 89-96