Protein Bands Detection by Nanoparticles after Paper Chromatography

Document Type : Research Paper


Nanotechnology group of Shahid Beheshti Medical University, Tehran, I. R. Iran Young Research Club, Pharmacy Faculty, Islamic Azad University of Medical Science, Tehran, I.R. Iran


Paper chromatography is an analytical technique for separating and identifying mixtures of materials. It is a useful technique since it is relatively quick, and requires small quantities of the material. By emergence of nanotechnology, paper chromatography has found many applications in biology and biotechnology. In this study, we employed gold and silver nanoparticles to detect protein bands after the paper chromatography method. We first performed paper chromatography on a solution containing Bovine Serum Albumin (BSA) protein. Then, the gold and silver nanoparticles were exploited for paper coloration. As a result, it was noticed that location of the protein bands was clearly distinct and detectable with this technique.