Biosynthesis Characteristic of Silver ‎Nanoparticles Produced by Mine Soil ‎Bacteria Isolation, Kerman, Iran

Document Type : Research Paper


1 ‎Department of Biology. Faculty of Science, Shahid ‎Bahonat University of Kerman, Kerman, Iran.‎

2 Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.‎

3 Young Researchers and Elite Club, Kerman Branch, Islamic Azad University, Kerman, Iran.‎


   Background: Biosynthesis of is one of the safe methods in nanoparticles (NP) production where local bacteria of every region can be applied for its better functionality. Among these nanoparticles, the silver nanoparticles (SNP) with high applicability due to their antimicrobial characteristic are the most common.
   Methods: In the study, the soil samples are collected from metal mines of Kerman province and their bacteria content is screened and purified. Then, the SNP biosynthesis test is run on Biomass and bacterial supernatant. The parameters here consist of two Molarity of silver nitrate (10-2 and 10-3), different temperature effect on biosynthesis of SNP, and light/dark conditions which are assessed here to optimize SNP production rate, and the results are confirmed.
   Results: From the bacterial isolations, the Pseudomonas Iranica NCBI: KF742672, was able to produce SNP from silver nitrate salt of 20-80 nm at 30°C, In dark condition and 10-2 Molarity.
   Conclusions: Bacteria can be consumed as a safe and clean element to produce NP, which is in wide use due to new characterizations that are revealed through studies, of course with the safety factor is of major concern.