International Journal of Nanoscience and Nanotechnology

International Journal of Nanoscience and Nanotechnology

Antioxidant Activity, Sun Protection Factor (SPF) and Total Phenolic and Flavonoid Contents from Purified Extract of Stelechocarpus buharol (BI.) Hook F. & Th. Leaves and its Classification with Chemometrics

Document Type : Research Paper

Authors
N. Nurhidayah
D. Diniatik
N. A. Nurulita
Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto P. O. Box 202, Banyumas, Indonesia
Abstract
   Stelechocarpus buharol (BI.) Hook F. & Th leaves were contains flavonoids and have antioxidant activity. This study determined the antioxidant activity, Sun Protection Factor (SPF) value, total phenolic and flavonoid content of ethanol extract (EESL) and purified extract of S. buharol leaves (PESL). EESL was obtained by maceration method and PESL by using n-hexane to remove the non-polar compound then obtain the purified ethanol extract. EESL and PESL were determined the antioxidant activity by using 1,2-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching assay (BCB) and classified by using chemometrics of principal component analysis (PCA). The results showed antioxidant activity with DPPH were IC50 value of vitamin C 4.59±0.046 µg/ml, EESL 9.53±0.062 µg/ml and PESL 5.95±0.048 µg/ml, with FRAP method were IC50 values of vitamin C 6.66±0.150 µg/ml, EESL 16.13±0.156 µg/ml and PESL 24.53±0.114 µg/ml and with BCB method were IC50 values of quercetin 236.67±8.808 µg/ml, EESL 134.25±4.478 µg/ml and PESL 116.82±9.982 µg/ml. The SPF value of PESL was 5.70. Total phenolic content of EESL was 5.68 ± 0.042% and PESL was 6.11 ± 0.020%, while the total flavonoid content of EESL 5.62 ± 0.006% and PESL were 6.67 ±0.017%. Based on the results of the study it can be concluded that the results of the measurement of antioxidant activity in EESL and PESL provide good antioxidant activity. The SPF of PSEL at a concentration of 200 ppm of 5.70. The total phenolic and total flavonoid contents correlated with DPPH, FRAP, and BCB. PCA classified EESL and PESL using the variables of antioxidant activities and phenolic-flavonoid contents.
Keywords
Stelechocarpus buharol leaves
Antioxidant activity
DPPH
BCB
FRAP
SPF
Total phenolic content
Total flavonoid content
Subjects
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International Journal of Nanoscience and Nanotechnology
Volume 18, Issue 3
Summer 2022
Pages 179-185