International Journal of Nanoscience and Nanotechnology

International Journal of Nanoscience and Nanotechnology

Preparation of Composites of Antibacterial ‎Materials Based on Bacterial Cellulose ‎and Silver Nanoparticles for Wound ‎Healing

Document Type : Research Paper

Authors
B. Rakhimova 1, 2
K. Kudaibergenov 1
L. Sassykova 1
G. Spanova 1
S. Aknazarov 1, 2
M. Tulepov 1
1 ‎Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, ‎‎050040, Almaty, Kazakhstan
2 Scientific and Production Technical Center “Zhalyn” LLP, 050012, Almaty, Kazakhstan
Abstract
   This study examined the preparation of composites of Bacterial Cellulose (BC) / silver nanoparticles (Ag NPs) using a substrate for the fermentation of the bacteria Gluconacetobacter xylinus C-3 and a reducing agent for the synthesis of silver nanoparticles in situ. The presence of silver in the BC/ Ag NPs composites was confirmed by various characterization tests. Thus, according to Scanning Electron Microscopy, the average size of silver was recorded at 700 nm. X-ray phase analysis showed an increase in the size of cellulose crystallites up to 122 Å with a decrease in the amount of silver nanoparticles. According to UV-visible spectroscopy, an identification peak was found between 420-580 nm. Measurements of the percentage of water absorption and the edge angles of wetting with water confirmed the structures of the resulting nanocomposites - with a smaller pore diameter and a narrow pore distribution over the film size, depending on the silver content. Using the disk diffusion method, excellent antimicrobial activity was shown with a decrease in the number of bacteria in relation to the pathogenic microorganism Staphylococcus (S.) aureus, Pseudomonas (P.) aeruginosa, Escherichia (E.) coli. 
Keywords
Antimicrobial activity
Bacterial cellulose
Composites
Hydrothermal synthesis
Silver nanoparticles
‎Turkevich citrate method.‎
Subjects
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International Journal of Nanoscience and Nanotechnology
Volume 18, Issue 2
Spring 2022
Pages 123-133