Role of Green Synthesized CuO ‎Nanoparticles of Trigonella Foenum-‎Graecum L. Leaves and their Impact on ‎Structural, Optical and Antimicrobial ‎Activity ‎

Document Type : Research Paper


1 PG & Research Department of Physics, Sadakathullah Appa College, Tirunelveli, India

2 Department of Physics, Coimbatore Institute of Technology, Coimbatore, India

3 Department of Mathematics, Karunya Institute of Technology and Sciences, Coimbatore, ‎India

4 Department of Life Sciences, Kristu Jayanti College (Autonomous), Bengaluru, India


   The present research work focused on the synthesis, characterization and testing of antimicrobial activity of CuO nanoparticles prepared by green synthesis method usingTrigonella foenum-graecum L. leaf extract. The XRD pattern indicated the presence of cubic structure of nanocrystalline CuO with crystallite size of 47 nm. The optical band gap was calculated using Tauc’s plot and found to be 3.6 eV. SEM images revealed that the particles had cluster structures with a combination of different shapes. EDAX analysis confirmed the presence of copper and oxide by indicating predominant peak which was the highest percentage present in the spectrum. The synthesized nanoparticles showed significant antibacterial and antifungal activity against the human pathogens Staphylococcus aureus and Penicillium sp. So this nanoparticle can be considered as an alternate approach for reducing the adhesion ofmicro-organisms.


Main Subjects

  1. Saranya, S., Vijayaranai, K., Pavithra, S., Raihana, N., Kumanan, K., “In vitro cytotoxicity of zinc oxide, iron oxide and copper nanopowders prepared by green synthesis”, Toxicol. Rep, 4 (2017) 427–430.
  2. Heydari, S., Shirmohammadi Aliakbarkhani, Z., Hosseinpour Zaryabi, M., “Photocatalytic Degradation of Safranin Dye from Aqueous Solution Using Nickel Nanoparticles Synthesized by Plant Leaves”, Int. J. Nanosci. Nanotechnol., 16 (2020) 153-165.
  3. Hadi, S.M., Bagherieh-Najjar, M.B., Kohan B.E., Mianabadi, M., “Ag-Conjugated Nanoparticle Biosynthesis Mediated by Rosemary Leaf Extracts Cor-rlates with Plant Antioxidant Activity and Protein Content”, Int. J. Nanosci. Nanotechnol., 14 (2018) 319-325.
  4. Ravichandiran, C., Sakthivelu, A., Davidprabu, R., Valanarasu, S., Kathalingam, A., Ganesh, V., Mohd. Shkir, Algarni, H., AlFaify, S., “In-depth study on structural, optical, photoluminescence and electrical properties of electrodeposited Cu2O thin films for optoelectronics: An effect of solution pH”, Microelectron. Eng, 210 (2019) 27–34.
  5. Lu, Q., Wang, X., Yu, J., Feng, F., Yin, L., Kang, Y., Luo, H., “Synthesis of spindle-like CuO nanoparticles by using cathode glow discharge electrolysis plasma”, Mater. Lett, 264 (2020) 127316.
  6. Duraipandi Devi Priya, Selvaraj Mohana Roopan, Sehajpreet Singh, Jatin Bansal, Shajahan Shanavas, Maksudur Rahman Khan, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Veeramuthu Duraipandiyan, “Phyto-synthesis of CuO nano-particles and its catalytic application in C-S bond Formation”, Mater. Lett., 266 (2020) 127486.
  7. Arunkumar, B., Johnson Jeyakumar, S., Jothibas, M., “A sol-gel approach to the synthesis of CuO nanoparticles using Lantana camara leaf extract and their photo catalytic activity”, Optik - International Journal for Light and Electron Optics, 183 (2019) 698–705.
  8. Davarpanah, S.J., Karimian, R., Goodarzi, V., Piri, F., “Synthesis of Copper (II) Oxide (CuO) Nanoparticles and Its Application as Gas Sensor”, Appl. Biotechnol. Rep, 2 (2015) 329-332.
  9. Paseban, N., Ghadam, P., Pourhosseini, P.S., “The Fluorescence Behavior and Stability of AgNPs Synthesized by Juglans Regia Green Husk Aqueous Extract”, Int. J. Nanosci. Nanotechnol., 15 (2019) 117-126.
  10. Mirzaei, S., Ghabooli, A., Mirzaei, M., “Botrytis Cinerea, One of the Most Destructive Plant Pathogens, as a Potent to Produce Silver Nanoparticles”, Int. J. Nanosci. Nanotechnol, 16 (2020) 243-248.
  11. Dudhane, A. A., Waghmode, S. R., Dama, L. B., Mhaindarkar, V. P., Sonawane  A., Katariya, S., “Synthesis and Characterization of Gold Nanoparticles using Plant Extract of Terminalia arjuna with Antibacterial Activity”, Int. J. Nanosci. Nanotechnol, 15 (2019) 75-82.
  12. Pal, S., Mondal, S., Maity, J., Mukherjee, R. “Synthesis and characterization of ZnO nanoparticles using Moringa oleifera leaf extract: Investigation of photocatalytic and antibacterial activity”, Int. J. Nanosci. Nanotechnol, 14 (2018) 111-119.
  13. Ananda Murthy, H.C., Buzuayehu Abebe, Tegene Desalegn, Z., Prakash, C.H., Kumar Shantaveerayya, “A Review on Green Synthesis and Applications of Cu and CuO Nanoparticles”, Mat. Sci. Res. India, 15(3), (2018) 279-295.
  14. Phukan, S., Mahanta, A., Kakati, D., Rashid, M. H., “Green chemical synthesis of Pd nanoparticles for use as efficient catalyst in Suzuki-Miyaura cross-coupling reaction”, Appl. Organomet. Chem., 33 (2019) e4758.
  15. Phukan, S., Kakati, D., Rashid, M. H., “Use of invasive weed to synthesize shape-tunable gold nanoparticles and evaluation of their catalytic activities in dye reduction”, Curr. Nanosci., 14 (2018) 511–519.
  16. Gmach, J., Joachimiak, Ł., Błazewska, K. M., “aza-Michael addition of imidazole analogues”, Synthesis, 48 (2016)  2681– 2704.
  17. Chowdhury, R., Mollick, M. M. R., Biswas, Y., Chattopadhyay, D., Rashid, M. H., “Biogenic synthesis of shape-tunable Au-Pd alloy nanoparticles with enhanced catalytic activities”, J. Alloys Compd, 763 (2018) 399–408.
  18. Arunkumar, B., Johnson Jeyakumar, S., Jothibas, M., “A sol-gel approach to the synthesis of CuO nanoparticles using Lantana camara leaf extract and their photo catalytic activity”, Optik - International Journal for Light and Electron Optics, 183 (2019) 698–705.
  19. Raja Naika, H., Lingaraju, K., Manjunath, K., Danith Kumar., Nagaraju, G., Suresh, D., Nagabhushana, H., “Green synthesis of CuO nanoparticles using Gloriosa superba L.extract and their antibacterial activity”, Journal of Taibah University for Science, 9 (2015) 7–12.
  20. Muthuvel, A., Jothibas, M., Manoharan, C., “Synthesis of copper oxide nanoparticles by chemical and biogenic methods: photocatalytic degradation and in vitro antioxidant activity”, Nanotechnology for Environmental Engineering, 5:14 (2020) 1-19.
  21. Keabadile , O. P., Aremu, A. O., Elugoke, S. E., Fayemi, O. E., “Green and Traditional Synthesis of Copper Oxide Nanoparticles-Comparative Study”, Nanomaterials, 10 (2020)  2502.
  22. Zhang, D., Ni, X., Zheng, H., Li, Y., Wang, G., Yang, Z., “Synthesis of needle-like nickel nanoparticles in water-in-oil microemulsion”, Mater. Lett, 59 (2005) 2011–2014.
  23. Rakesh Chowdhury., Aslam Khan., Md. Harunar Rashid., “Green synthesis of CuO nanoparticles using Lantana camara flower extract and their potential catalytic activity towards the aza-Michael reaction”, RSC Advances, 10 (2020) 14374.
  24. Chieu D. Tran., James Makuvaza., Erik Munson., Brian Bennett., “Biocompatible Copper Oxide Nanoparticle Composites from Cellulose and Chitosan: Facile Synthesis, Unique Structure, and Antimicrobial Activity”, ACS Applied Materials & Interfaces, 9:49 (2017) 42503-42515.
  25. Biswajoy Bagchi., Sumit Dey., Suman Bhandary., Sukhen Das., Alakananda Bhattacharya., Ruma Basu., Papiya Nandy., “Antimicrobial efficacy and biocompatibility study of copper nanoparticle adsorbed mullite aggregates”, Materials Science and Engineering C, 32 (2012) 1897–1905.
  26. Mittal, D., Narang, K., Leekha Kapinder, A., Kumar, K., Verma, A. K., “Elucidation of Biological Activity of Silver Based Nanoparticles Using Plant Constituents of Syzygium cumini”, Int. J. Nanosci. Nanotechnol, 15 (3), (2019) 189-198.
  27. Brajesh Kumar., Kumari Smita., Luis Cumbal., Alexis Debut., Yolanda Angulo., “Biofabrication of copper oxide nanoparticles using Andean blackberry (Rubus glaucus Benth.) fruit and leaf”, Journal of Saudi Chemical Society, 21 (2017) S475–S480.
  28. Albert manoharan, A., Chandramohan, R., David prabu, R., Valanarasu, S., Ganesh, V., Shkir, M., Kathalingam, A., AlFaify, S., “Facile synthesis and characterization of undoped, Mn doped and Nd co-doped CuO nanoparticles for optoelectronic and magnetic applications”, J. Mol. Struct, 1171 (2018) 388-395.
  29. Hepzi Pramila Devamani, R., Alagar, M., “Synthesis and Characterisation of Copper II Hydroxide Nano Particles”, Nano Biomed. Eng, 5 (3), (2013) 116-120.
  30. Mote, V.D., Purushotham, Y., Dole, B.N., “Williamson-Hall analysis in estimation of lattice strain in nanometer-sized ZnO particles”, Journal of Theoretical and Applied Physics, 6 (2012) 6.
  31. Johnsy Arputhavalli, G., Agilan, S., Mahithosh Ray., Thangaraju, D., Marnadu, R., Chandrasekaran, J., Chandekar, K.V., Mohd. Shkir., Jebasingh, S., Dinesh, M., “Post-annealing effects on structural and magnetic properties of pulsed laser deposition grown Co–Ni–Al ferromagnetic shape memory alloys thin films”, Solid state sciences, 111 (2021) 106493.
  32. Dhineshbabu, N. R., Rajendran, V., Nithyavathy, N., Vetumperumal, R., “Study of structural and optical properties of cupric oxide Nanoparticles”, Appl Nanosci, 6 (2016) 933–939.
  33. Huang, C.Y., Chatterjee, A., Liu, S.B., Wu, S.Y., Cheng, C.L., “Photoluminescence properties of a single tapered CuO nanowire”, Appl. Surf. Sci, 256 (2010) 3688–3692.
  34. Anshuman Sahai., Navendu Goswami., Kaushik, S.D., Shilpa Tripathi., “Cu/Cu2O/CuO nanoparticles: Novel synthesis by exploding wiretechnique and extensive characterization”, Appl. Surf. Sci, 390 (2016) 974–983.
  35. Ansar Mehmood., Ghulam Murtaz., Tariq Mahmood Bhatti., Rehana Kausar., “Phyto-mediated synthesis of silver nanoparticles from Melia azedarach L. leaf extract: Characterization and antibacterial activity”, Arab. J. Chem, 10 (2017) S3048–S3053.
  36. Reda Hassanien., Dalal Z. Husein., Mostafa F. Al-Hakkani., “Biosynthesis of copper nanoparticles using aqueous Tilia extract: antimicrobial and anticancer activities”, Heliyon, 4 (2018) e01077.
  37. Saranya Sukumar., Agneeswaran Rudrasenan., Deepa Padmanabhan Nambiar., “Green-Synthesized Rice-Shaped Copper Oxide Nanoparticles Using Caesalpinia bonducella Seed Extract and Their Applications”, ACS Omega, 5 (2020) 1040−1051.
  38. Faheem Ijaz., Sammia Shahid., Shakeel Ahmad Khan., Waqar Ahmad., Sabah Zaman., “Green synthesis of copper oxide nanoparticles using Abutilon indicum leaf extract: Antimicrobial, antioxidant and photocatalytic dye degradation activities”, Tropical Journal of Pharmaceutical Research, 16 (4) (2017) 743-753.
  39. Gomathi, M., Rajkumar, P.V., Prakasam, A., Ravichandran, K., “Green synthesis of silver nanoparticles using Datura stramonium leaf extract and assessment of their antibacterial activity”, Resource-Efficient Technologies, 3 (2017) 280–284.
  40. Shibana, A.T., Raiza, J., Pandian, K., “Amido-Amino Clay Stabilized Copper Nanoparticles: Antimicrobial Activity and Catalytic Efficacy for Aromatic Amination”, Int. J. Nanosci. Nanotechnol, 16 (2020) 117-125.
  41. Nallendran, R., Selvan, G., Balu, A.R., “NiO coupled CdO nanoparticles with enhanced magnetic and antifungal Properties”, Surfaces and Interfaces, 15 (2019) 11–18.
  42. Vala, A. K., Shah, S., “Rapid Synthesis of Silver Nanoparticles by a Marine-derived Fungus Aspergillus Niger and their Antimicrobial Potentials”, Int. J. Nanosci. Nanotechnol, 8 (4), (2012) 197-206.