Green Synthesis of Silver Nanoparticles and Its Effect on Total Proteins in Melia Azedarach Plant

Document Type: Research Paper

Authors

1 Department of Plant Breeding, Faculty of Agricultureal, Shahid Bahonar University of Kerman, Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, I. R. Iran

2 Department of Biotechnology, Faculty of Agricultureal, Shahid Bahonar University, Kerman, I. R. Iran

3 Department of Engineer Chemistry, Faculty of Engineering, Shahid Bahonar University, Kerman, I. R. Iran

Abstract

Silver nano-particles have different biological applications due to their compatibility. Chemical methods usually result in remaining some amounts of toxic reactions on the nano-particles. For this reason, the use of plants as sustainable and accessible resources in preparation of compatible nano-particles has attracted a lot of attention in the recent years. This research investigated the green synthesis of Silver nano-particles using Meliaazedarach plants.The analysis of Ultraviolet–visible spectroscopyshowed the green synthesis of nanoparticles in plants. X-ray diffraction was used to confirm the crystalline nature of the particles. Fouriertransform infrared spectroscopyspectroscopywas performed to analyzethe functional groupin the process of green synthesis, the role of different functional groups such as hydroxyl, amine and alkyl groups were indicated in the synthetic process.Then, proteinscapped on the silver nano-particles were analyzed usingSodium dodecyl sulfate -PAGE. Regarding this, the possibility of extracted protein role in the stabilization of nano-particles requires more analysis.

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