Botrytis Cinerea, One of the Most ‎Destructive Plant Pathogens, as a Potent ‎to Produce Silver Nanoparticles

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University,‎ Hamedan, Iran.‎

2 Department of Materials Science and Engineering, School of Engineering, Shiraz University, ‎Shiraz, Iran.‎

Abstract

   Nanoparticles are synthesized using different physical and chemical methods. However, the development of an eco-friendly approach for the synthesis of nanoparticles is of critical importance to nanotechnology. Types of fungi which secrete a high amount of proteins are ideal candidates for the eco-friendly synthesis of nanoparticles. In this research, the extracellular biosynthesis of silver nanoparticles was implemented, using Botrytis cinerea. UV-vis spectroscopy illustrated a sharp peak at 420 nm, demonstrating the presence of silver nanoparticles in the fungal cell filtrate. Further analysis was accomplished through TEM and FTIR. Silver nanoparticles were spherical and 5.1-13.95 nm in diameter with an average size of 8.55 nm. NPs were stable three months after their formation, which is, quite likely, due to their capping with proteins which were secreted by the fungus.

Keywords

References

  1. Kyriacou, S., V., Brownlow, W. J., Xu, X. H., “Using nanoparticle optics assay for direct observation of the function of antimicrobial agents in single live bacterial cells”, Biochemistry, 43 (2004) 140–147.
  2. Saha, S., Chattopadhyay, D., Acharya, K., “Preparation of silver nanoparticles by bio-reduction using nigrospora oryzae culture filtrate and its antimicrobial activity”, Dig. J. Nanomater. Biostructures, 6 (2011) 1519–1528.
  3. Rai, M., Yadav, A., Gade, A., “Silver nanoparticles as a new generation of antimicrobials”, Biotechnol. Adv., 27 (2009) 76–83.
  4. Pal, S., Tak, Y. K., Song, J. M., “Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli”, Appl. Environ. Microbiol., 73 (2007) 1712–1720.
  5. Rai, M. K., Deshmukh, S. D., Ingle, A. P., Gade, A. K., “Silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria”, J. Appl. Microbiol., 112 (2012) 841–852.
  6. Marambio-Jones, C., Hoek, E. V., “A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment”, J. Nanopart. Res., 12 (2010) 1531–1551.
  7. Velhal, S. G., Kulkarni, S. D., Latpate, R. V., “Fungal mediated silver nanoparticle synthesis using robust experimental design and its application in cotton fabric”, Int. Nano Lett., 6 (2016) 257–264.
  8. Naveen, H. K. S., Kumar, G., Karthik, L., Bhaskara Rao, K. V., “Extracellular biosynthesis of silver nanoparticles using the filamentous fungus Penicillium sp.”, Arch. Appl. Sci. Res., 2 (2010) 161–167.
  9. Vala, A., Shah, S., “Rapid synthesis of silver nanoparticles by a marine-derived fungus Aspergillus niger and their antimicrobial potentials”, Int. J. Nanosci. Nanotechnol., 8 (2012) 197–206.
  10. Sastry, M., Ahmad, A., Khan, M.  I., Kumar, R., “Biosynthesis of metal nanoparticles using fungi and actinomycetes”, Cur. Sci., 85 (2003) 162–170.
  11. Mandal, D., Bolander, M. E., Mukhopadhyay, D., Sarkar, G., Mukherjee, P., “The use of microorganisms for the formation of metal nanoparticles and their application”, Appl. Microbiol. Biotechnol., 69 (2006) 485–492.­
  12. Rai, M., Yadav, A., Gade, A., “Mycofabrication, mechanistic aspect and Multifunctionality of Metal Nanoparticles - Where are we? And where should we go?’, in Mendez-Vilas, A. (ed.) Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Badajoz, Spain: Formatex Research Center, (2010) pp. 1343–1354.
  13. Sriramulu, M., Sumathi, S., “A mini review on fungal based synthesis of silver nanoparticles and their antimicrobial activity”, Int. J. Chem.Tech. Res., 10 (2017) 367–377.
  14. Jarvis, W. R., “Botryotinia and Botrytis species: taxonomy, physiology and pathogenicity-A guide to the literature”, Agriculture Canada, (1977). 
  15. van Kan,  J. A. L., “Licensed to kill: the lifestyle of a necrotrophic plant pathogen”, Trends Plant Sci., 11 (2006) 247–253.
  16. Bhainsa, K. C., D’Souza, S. F., “Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus”, Colloids Surf. B Biointerfaces, 47 (2006) 160–164.
  17. Ahmad, A., Mukherjee, P., Senapati, S., Mandal, D., Khan, M. I. I., Kumar, R., Sastry, M., “Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum”, Colloids Surf. B Biointerfaces, 28 (2003) 313–318.
  18. Natarajan, K., Selvaraj, S., Murty, V. R., “Microbial production of silver nanoparticles”, Dig. J. Nanomater. Biostructures, 5 (2010) 135–140.
  19. Li, G., He, D., Qian, Y., Guan, B., Gao, S., Cui, Y., Yokoyama, K., Wang, L., “Fungus-mediated green synthesis of silver nanoparticles using Aspergillus terreus”, Int. J. Mol. Sci., 13 (2012) 466–476.
  20. Mukherji, S., Bharti, S., Shukla, G., Mukherji, S., “Synthesis and characterization of size- and shape-controlled silver nanoparticles”, Phys. Sci. Rev., 4 (2018) 1–73.
  21. Radhi, M. M., Moosa, A. A., Khalaf, I. A., “Performance Improvement of Working Electrode Using Grafted Polymer Modified with SiO2 Nanoparticles”, Nano Biomed. Eng., 10 (2018) 156-164
  22. Ghaseminezhad,  S. M., Hamedi, S., Shojaosadati, S. A., “Green synthesis of silver nanoparticles by a novel method: Comparative study of their properties”, Carbohydr. Polym., 89 (2012) 467–472.
  23. Gole, A., Dash, C., Ramakrishnan, V., Sainkar, S. R. , Mandale, A. B., Rao, M., Sastry, M.,  “Pepsin−Gold Colloid Conjugates:  Preparation, Characterization, and Enzymatic Activity”, Langmuir, 17 (2001) 1674–1679.
  24. Mohanpuria, P., Rana, N. K., Yadav, S. K., “Biosynthesis of nanoparticles: technological concepts and future applications”, J. Nanoparticle Res., 10 (2008) 507–517.
International Journal of Nanoscience and Nanotechnology
Volume 16, Issue 4
November 2020
Pages 243-248

Files

Share

How to cite

Statistics