Iranian Nanotechnology Society International Journal of Nanoscience and Nanotechnology 1735-7004 10 3 2014 09 01 Iron Nanoparticles and Methionine Hydroxy Analogue Chelate in ovo Feeding of Broiler Chickens 187 196 9102 EN A. A. Saki Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, I. R. Iran M. Abbasinezhad Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, I. R. Iran A. A. Rafati Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, Hamedan, I. R. Iran Journal Article 2014 01 29 The 440 fertile eggs, in a completely randomized design were divided into 11 treatments with four replicates and 10 eggs per each replicate. Treatments including: (T1; control; without injection), (T2; injected with 0.3 ml saline 9.0%; sham control), (T3, T4 and T5) 25, 75, 125 ppm Fe-Nano, (T6, T7 and T8) 50, 100, 150 ppm Fe-Nano-Alimet chelate, (T9, T10 and T11) 50, 100, 150 ppm Fe-Aliment chelate. On 10th day of incubation, 0.3 ml solution in ovo was injected into the egg yolk sac. Higher hatchability was found in controls, 25 ppm Fe- Nano, 100 ppm Fe-Nano-Alimet chelate and all levels of Fe-Alimet chelate treatments than other treatments. The egg weight was higher significantly in sham control, 25 and 75 ppm Fe-Nano, all levels of Fe-Nano-Alimet chelate and 150 ppm Fe-Aliment chelate treatments. There was significant increase in chick weight in controls, 25 and 75 ppm Fe-Nano, 100 ppm Fe-Nano-Alimet chelate and 150 ppm Fe-Aliment chelate in comparison with other treatments. Also chick body weight to egg weight ratio in controls, 25ppm Fe-Nano and 100ppm Fe- Nano-Alimet chelate was higher than in other treatments. Level of 25 ppm of Fe-Nano has shown the highest relative liver weight. Serum Fe content and liver was higher by using 25 ppm Fe-Nano, 100 ppm Fe-Nano- Alimet chelate and 150 ppm Fe-Aliment chelate than other treatments. By applying two treatments of 100 ppm Fe-Nano-Alimet chelate and 150 ppm Fe-Aliment chelate, chickens blood hemoglobin increased significantly compared with the other treatments. These results suggest that 25 ppm Fe-Nano, 100 ppm Fe-Nano-Alimet chelate and 150 ppm Fe-Alimet chelate improved embryonic growth and development. https://www.ijnnonline.net/article_9102_ff1d4796fe85a21ba86081db7bf2196b.pdf