Iranian Nanotechnology Society International Journal of Nanoscience and Nanotechnology 1735-7004 21 2 2025 06 30 Nano Chitosan-Paracetamol Composite as a Potential Lung Cancer Inhibitor: Synthesis, Characterization, and Cytotoxicity Studies. 59 66 737959 10.22034/ijnn.2026.2053411.2633 EN Bahaa K. Al-Ghanimi Department of Anaesthesia and Critical Care, Al-Taff University College, Kerbala 56001, Iraq. 0000-0003-1369-063X Ousama W. Abdulnabi University of Kerbala/ College of education for pure sciences/ Department of chemistry 0009-0000-5228-9932 Hussein H. Al-Ghanimi Biomedical Engineering Department, College of Engineering, University of Warith Al-Anbiyaa, Karbala 56001, Iraq. 0000-0003-2973-393X Mohammad N. AL-Baiati Department of Chemistry, College of Education for Pure Sciences, University of Kerbala, Karbala, 56001, Iraq. 0000-0001-6947-2629 Khawla I. Abd Nusaif College of Veterinary Medicine, University of Kerbala, Karbala, 56001. Iraq Journal Article 2025 02 13 Nanoparticles have been used in many areas of life, including medicine, due to the properties they possess that distinguish them from conventional particles. In the current study, Nano chitosan-paracetamol was synthesized, and the synthesized nanoparticles were characterized using FT-IR and 1H-NMR techniques. A drug was created using a variety of properties, and its anticancer properties against lung cancer A549 cell lines were investigated in vitro. It was determined what kinds of linkages existed between amino acids and nano-chitosan medications. The outcomes demonstrated how well the medications work to stop cancer cells from spreading. The effect of nano-chitosan on the toxicity of lung cancer cells (A549) was shown only with an IC50 = 137.87 µg/ml. However, when paracetamol was loaded onto nano-chitosan, better results were achieved, with a toxicity of lung cancer cells (A549) with an IC50 = 109.49 µg/ml. The result of gene expression of p53 gene for the control group was (1.00 + 0.0 N=3) and when exposed to nano chitosan only, a significant mutation appeared and the result was (2.933 + 0.2028 N=3) but when exposed to the prepared nano drug, the result became (4.100 + 0.1528 N=3). The rationale is the pressing requirement that the medication maintain its benefits for an extended duration until it more effectively reaches particular body parts without endangering other body parts. When the synthesis of Nano-Chitosan-Paracetamol was tested for biological activity, it was found to be highly successful in preventing the lung cancer cells from spreading quickly. https://www.ijnnonline.net/article_737959_3366c88e99b8b4cfbcb27cf0d4f44fc7.pdf