Iranian Nanotechnology Society International Journal of Nanoscience and Nanotechnology 1735-7004 20 2 2024 05 31 Purified Compound of the Seeds of Carica Papaya Loaded mPEG-PLA Polymeric Nanomicelle (MCPI-PNs) for Reproductive Studies in Albino Male Rats 77 90 715064 10.22034/ijnn.2024.2007929.2418 EN Abdul S. Ansari Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, Rajasthan 302004, India 0000-0001-5244-5069 Rajeev Kumar Dhaked Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, Rajasthan 302004, India Barkha Khilwani Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, Rajasthan 302004, India Nirmal K. Lohiya Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, Rajasthan 302004, India Journal Article 2023 08 23 <em>   This study compares the characterized MCP I-loaded methoxy poly (ethylene glycol)-poly (lactic acid) polymeric nanomicelles (MCPI-PNs) of different sizes and doses to find out the maximum antireproductive effects with purified compound of seeds of Carica papaya. The prepared MCPI-PNs consisting of six groups (n=36), viz., vehicle treated control (group 1), alone MCP I @ 10 mg/animal/day (group 2), 46.03 nm sized blank mPEG-PLA nanomicelles @ 0.3 mL/animal/day (group 3), 100 nm sized MCPI-PNs (1.5, 3.0 and 4.5 µg/animal/day) (group 4), 129 nm sized MCPI-PNs (4.5, 9.0 and 13.5 µg/animal/day) (group 5) and 166 nm sized MCPI-PNs (13.5, 27.0 and 45.0 µg/animal/day) (group 6) were orally treated for a period of 30 days in albino male rats. Animals of groups 2, 4, 5 and 6 showed significantly altered sperm parameters. However, the sperm concentration maximally dropped in group 2 and 4 (low dose and mid dose), respectively, indicating the range of oligozoospermia to severe oligozoospermia in these groups. The fertility declined to zero percent in groups 2 and 4 (low dose), whereas all animals were 100% fertile in rest of groups. Histopathological studies of testes exhibited sloughing and eruption of germ cells with inhibition of spermatogenesis in groups 2 and 4, whilst some conspicuous alterations in spermatogenesis was observed in groups 5 and 6 when compared to groups 1 and 3. Based on the above observations, the group 4 (100 nm sized MCPI-PNs) at low dose regimen (1.5 µg/animal/day) exhibited maximum antifertility effects in male albino rats.</em> https://www.ijnnonline.net/article_715064_73040ccd2aaca75933c5afc9c0122db3.pdf