Iranian Nanotechnology Society International Journal of Nanoscience and Nanotechnology 1735-7004 10 2 2014 06 01 Green Synthesis of Silver Nanoparticles:Eco-Friendly and Antibacterial 127 132 6118 EN S. Shams Department of Plant Breeding, Faculty of Agricultureal, Shahid Bahonar University of Kerman, Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, I. R. Iran Sh. Pourseyedi Department of Biotechnology, Faculty of Agricultureal, Shahid Bahonar University, Kerman, I. R. Iran H. Hashemipour Rafsanjani Department of Engineer Chemistry, Faculty of Engineering, Shahid Bahonar University, Kerman, I. R. Iran Journal Article 2014 01 26 Nano world daily. In this paper the Lens culinaris seed extract was assessed for thegreen synthesis of silvernanoparticlesat temperature of 25◦C. The nanoparticles were characterized using Inductively CoupledPlasma spectrometry (ICP), X-ray diffraction (XRD) and Transmission electron microscopy (TEM). Thenthe antimicrobial activities of these nanoparticles were investigated. P. aeruginosa and S aureus were used as representatives of Gram-negative and Gram-positive bacteria, respectively. Percentage conversion of metal ion to metal nanoparticles were more than 95% after 60 days of the reaction. XRD was used to confirm the crystalline nature of the particles. Silver nanoparticles were mostly spherical with range in size from 5-25 nm. Ag nanoparticles synthesized with extract were exhibited a strong antibacterial activity against bothP.aeruginosa and S.aureus.This process is completely green and eco-friendly compatible.

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