Iranian Nanotechnology Society International Journal of Nanoscience and Nanotechnology 1735-7004 18 3 2022 08 01 Antioxidant Activity, Sun Protection Factor (SPF) and Total Phenolic and Flavonoid Contents from Purified Extract of Stelechocarpus buharol (BI.) Hook F. & Th. Leaves and its Classification with Chemometrics 179 185 254342 EN N. Nurhidayah Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto P. O. Box 202, Banyumas, Indonesia D. Diniatik Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto P. O. Box 202, Banyumas, Indonesia 0000-0002-2852-6739 N. A. Nurulita Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto P. O. Box 202, Banyumas, Indonesia Journal Article 2021 05 08 Stelechocarpus buharol (BI.) Hook F. & Th leaves were contains flavonoids and have antioxidant activity. This study determined the antioxidant activity, Sun Protection Factor (SPF) value, total phenolic and flavonoid content of ethanol extract (EESL) and purified extract of S. buharol leaves (PESL). EESL was obtained by maceration method and PESL by using n-hexane to remove the non-polar compound then obtain the purified ethanol extract. EESL and PESL were determined the antioxidant activity by using 1,2-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching assay (BCB) and classified by using chemometrics of principal component analysis (PCA). The results showed antioxidant activity with DPPH were IC50 value of vitamin C 4.59±0.046 µg/ml, EESL 9.53±0.062 µg/ml and PESL 5.95±0.048 µg/ml, with FRAP method were IC50 values of vitamin C 6.66±0.150 µg/ml, EESL 16.13±0.156 µg/ml and PESL 24.53±0.114 µg/ml and with BCB method were IC50 values of quercetin 236.67±8.808 µg/ml, EESL 134.25±4.478 µg/ml and PESL 116.82±9.982 µg/ml. The SPF value of PESL was 5.70. Total phenolic content of EESL was 5.68 ± 0.042% and PESL was 6.11 ± 0.020%, while the total flavonoid content of EESL 5.62 ± 0.006% and PESL were 6.67 ±0.017%. Based on the results of the study it can be concluded that the results of the measurement of antioxidant activity in EESL and PESL provide good antioxidant activity. The SPF of PSEL at a concentration of 200 ppm of 5.70. The total phenolic and total flavonoid contents correlated with DPPH, FRAP, and BCB. PCA classified EESL and PESL using the variables of antioxidant activities and phenolic-flavonoid contents.

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