Iranian Nanotechnology Society International Journal of Nanoscience and Nanotechnology 1735-7004 12 4 2016 11 01 Biogenic Approach using Sheep Milk for the Synthesis of Platinum Nanoparticles: The Role of Milk Protein in Platinum Reduction and Stabilization 199 206 24539 EN M. Gholami-Shabani Department of Mycology, Pasteur Institute of Iran, Tehran 13164, Iran Z. Gholami-Shabani Faculty of Aerospace, Science and Research Campus, Islamic Azad University, Tehran, Iran M. Shams-Ghahfarokhi Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115-331, Iran A. Akbarzadeh Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran 13164, Iran Gh. Riazi Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran M. Razzaghi-Abyaneh Department of Mycology, Pasteur Institute of Iran Journal Article 2014 12 31 Bio-directed synthesis of nanoparticles is an interesting field of rapid advancement for biologists, chemists and materials scientists, especially in light of efforts to find out green methods of inorganic material synthesis. In the present study, green synthesis of platinum nanoparticles (PtNPs) using sheep milk is reported for the first time. By adjusting the concentrations of chloroplatinic acid (H2PtCl6) and milk in aqueous solutions, spherical PtNPs were obtained at room temperature. The nanoparticles obtained were characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), high-resolution Transmission Electron Microscopy (TEM) and X-Ray diffraction (XRD). The spherical particles obtained have an average size 9.0 nm as shown by XRD pattern and TEM analysis. Fourier Transform Infra-Red (FTIR) measurements were carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the PtNPs synthesized by milk. The carboxylic acid group libration and amide I and II bands revealed the binding of protein with platinum surface through the amine group rather than the carboxyl group. Among a wide variety of biological resources which are introduced for reducing Pt ions into PtNPs, green approaches like using sheep milk have outstanding benefits for synthesize biological metal nanoparticles due to their eco-friendly phenomenon and very less amounts of cytotoxicity. PtNPs green synthesized in this study are potential candidates to use in drug discovery and gene transfer technologies.

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