Ag-Conjugated Nanoparticle Biosynthesis Mediated by Rosemary Leaf Extracts Correlates with Plant Antioxidant Activity and Protein Content

Document Type : Research Paper


1 Department of Biology, Golestan University, Shahid Beheshti Ave., P. O. Box: 155, Gorgan, I. R. Iran.

2 Department of Biology, Hakim Sabzevari University, P. O. Box: 397, Sabzevar, I. R. Iran.


   In recent years, the world has witnessed an explosion of interest in biogenic synthesis of metallic nanoparticles (NPs), using plant extracts; however, the plant constituents involved in this reaction are poorly characterized. Here, major components of Rosemary (Rosmarinus officinalis L.) leaf extracts were isolated monthly during 2012-2013 and their competences for Ag–conjugated nanoparticle biosynthesis were studied. Formation of Ag/AgCl–NPs was examined using UV-visible spectroscopy and characterized by Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), and Transmission electron microscopy (TEM). Correlation analysis showed that extracts with more protein contents and higher total reducing capacity were more efficient in biosynthesis of Ag/AgCl–NPs. There existed no correlation between total phenolic, ascorbic acid and anthocyanin contents with Ag/AgCl–NPs biosynthesis activity. These results partly explain the mechanisms controlling plant mediated biosynthesis of nanoparticles, with possible applications in standardization of plant materials harvested throughout a year.