Leucaena Leucocephala Mediated Green Synthesis of Silver Nanoparticles and Their Antibacterial, Dye Degradation and Antioxidant Properties

Document Type : Research Paper

Authors

Green Medicinal Chemistry Laboratory, Department of Chemistry, Osmania University, Hyderabad-500007, Telangana, India

Abstract

   The current study demonstrates a green, quick and easy method for producing and characterization of silver nanoparticles from dehydrated leaf extract of Leucaena leucocephala (Lam-AgNPs) to evaluate its antibacterial activity against harmful bacteria, photocatalytic degradation of dyes used in textile dyeing industries and antioxidant activity to reduce oxidative stress. The UV-Visible spectra maximum absorption peak (λmax) of the Lam-AgNPs was noticed at 436 nm. FTIR vibrational spectra revealed the phytoconstituents that cause the reduction and stabilization of AgNPs from the plant extract. The average crystallite size was calculated to be 26 nm using the Debye-Scherrer equation with X-ray diffraction studies. The EDS spectra screening exposed a high concentration of silver with a weight % of 71.67 % and atomic % of 30.49 %. TEM studies showed the nano-sized particles that were spherical to quasi-spherical in shape and polydistributed in nature. Most particles are spread in the 15-20 nm and 30-35 nm size ranges. Lam-AgNPs had effective antibacterial action against four tested strains such as Staphylococcus aureus, Bacillus subtilis, Pseudomonas putida and Klebsiella pneumoniae by the zone of inhibition observed at 20 mm, 19 mm, 15 mm and 16 mm, respectively. The DPPH free radical scavenging assay was used to study the antioxidant activity of Lam-AgNPs and the IC50 value calculated was 240.70µg/ml. In addition, the photocatalytic activity of biogenic Lam-AgNPs for the degradation of Red m5b dye was investigated under solar light irradiation and 100% degradation was noticed after 90 minutes of reaction.

Keywords

Main Subjects

References

  1. Meng, H., Liong, M., Xia, T., Li, Z., Ji, Z., Zink, J. I., Nel, A. E., “Engineered design of mesoporous silica nanoparticles to deliver doxorubicin and P-glycoprotein siRNA to overcome drug resistance in a cancer cell line”, ACS Nano, 4(8) (2010) 4539–4550.
  2. Yang, F., Jin, C., Subedi, S., Lee, C. L., Wang, Q., Jiang, Y., Li, J., Di, Y., Fu, D., “Emerging inorganic nanomaterials for pancreatic cancer diagnosis and treatment”, Treat. Rev, 38(6) (2012) 566–579.
  3. Mout, R., Moyano, D. F., Rana, S., Rotello, V. M., “Surface functionalization of nanoparticles for nanomedicine”, Soc. Rev, 41(7) (2012) 2539–2544.
  4. Mathew, T. V., Kuriakose, S., “Studies on the antimicrobial properties of colloidal silver nanoparticles stabilized by bovine serum albumin”, Surf. B Biointerfaces, 101 (2013) 14-18.
  5. Bindhu, M. R., Umadevi, M., “Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity”, Acta. A, 101 (2013) 184–190.
  6. Tamuly, C., Hazarika, M., Borah, S. C., Das, M. R., Boruah, M. P., “In situ biosynthesis of Ag, Au and bimetallic nanoparticles using Piper pedicellatum C.DC: green chemistry approach”, Colloids Surf. B. Biointerfaces, 102 (2013) 627–634.
  7. Rafiuddin, Z. Z., “Silver nanoparticles formation using tyrosine in presence acetyltrimethylammonium bromide”, Colloids Surf. B. Biointerfaces, 89 (2012) 211–215.
  8. Abebe, B., Ananda Murthy, H. C., Dessie, Y., “Synthesis and Characterization of Ti–Fe Oxide Nanomaterials: Adsorption–Degradation of Methyl Orange Dye”, Arabian Journal for Science and Engineering, 45(6) (2020) 4609–4620.
  9. Cheng, K., Hung, Y., Chen, C., Liu, C., Young, J., “Green synthesis of chondroitin sulfate-capped silver nanoparticles: characterization and surface modification”, Polym. 110 (2014) 195-202.
  10. Rajan, R., Chandran, K., Harper, S. L., Yun, S. I., Kalaichelvan, P. T., “Plant extract synthesized silver nanoparticles: An ongoing source of novel biocompatible materials”, Industrial Crops and Products, 70 (2015) 356–373.
  11. Jeyaraj, M., Sathishkumar, G., Sivanandhan, G., Mubarak Alid, D., Rajesh, M., Arun, R., Kapildev, G., Manickavasagam, M., Thajuddin, N., Premkumar, K., Ganapathi, A., “Biogenic silver nanoparticles for cancer treatment: an experimental report”, Colloids Surf. B. Biointerfaces, 106 (2013) 86–92.
  12. Singh, K. P., Singh, A. K., Gupta, S., Rai, P., “Modeling and optimization of reductive degradation of chloramphenicol in aqueous solution by zero-valent bimetallic nanoparticles”, Sci. Pollut. Res, 19(6) (2012) 2063–2078.
  13. Kumar, P. P. N. V., Pammi, S. V. N., Kollu, P., Satyanarayana, K. V. V., Shameem, U., “Green synthesis and characterization of silver nanoparticles using Boerhaavia diffusa plant extract and their antibacterial activity”, Crops Prod, 52 (2014) 562–566.
  14. Khan, M., Khan, S.T., Khan, M., Adil, S. F., Musarrat, J., Al-Khedhairy, A. A., Alkhathlan, H. Z., “Antibacterial properties of silver nanoparticles synthesized using Pulicaria glutinosa plant extract as a green bioreductant”, J. Nanomed, 9 (2014) 3551–3565.
  15. Rakgoshi, L., Blessing A. A., Shesan John O., Emanuel R., Youmbi T. F., Derek T. N., Suprakas S. R., “Gum Acacia/Carbopol-Based Biocomposites Loaded with Silver Nnanoparticles as Potential Wound Dressings”, J. Nanosci. Nanotechnol, 16(4) (2020) 219-231.
  16. Narayanan, K. B., Sakthivel, N., “Heterogeneous catalytic reduction of anthropogenic pollutant, 4-nitrophenol by silver-bionanocomposite using Cylindrocladium floridanum. Bioresour”, Technol, 102 (2011) 10737–10740.
  17. Kumar, P., Govindarajua, M., Senthamilselvi, S., Premkumar, K., “Photocatalytic degradation of methyl orange dye using silver (Ag) nanoparticles synthesised from Ulva lactuca”, Colloids Surf. B. Biointerfaces, 103 (2013) 658–661.
  18. Balavigneswaran, C. K., Sujin Jeba Kumar, T., Moses Packiaraj, R., Prakash, S., “Rapid detection of Cr(VI) by AgNPs probe produced by Anacardium occidentale fresh leaf extracts”, Nanosci, 4(3) (2014) 367–378.
  19. Elegbede, J., Lateef, A., “Nanotechnology in the built environment for sustainable development”, IOP Conference Series Materials Science and Engineering, 805(1) (2020) 012044.
  20. Agbaje, L., Elegbede, J. A., Akinola, P. O., Ajayi, V. A., “Biomedical applications of green synthesized-metallic nanoparticles: A review”, Pan African Journal of Life Sciences, 3(1) (2019) 157–182.
  21. Badmus, J. A., Oyemomi, S. A., Adedosu, O. T., Yekeen, T. A., Azeez, M. A., Adebayo, E. A., Lateef, A., Badeggi, U. M., Botha, S., Hussein, A. A., Marnewick, J. L., “Photo-assisted bio-fabrication of silver nanoparticles using Annona muricata leaf extract: Exploring the antioxidant, antidiabetic, antimicrobial, and cytotoxic activities”, Heliyon, 6(11) (2020) e05413.
  22. Aina, D. A., Owolo, O., Lateef, A., Aina, F. O., Hakeem, A. S., Adeoye-Isijola, M., Okon, V., Asafa, T. B., Elegbede, J. A., Olukanni, O. D., Adediji, I., “Biomedical Applications of Chasmanthera dependens stem extract mediated silver nanoparticles as Antimicrobial, Antioxidant, Anticoagulant, thrombolytic and Larvicidal agents, Karbala International Journal of Modern Science, 5(2) (2019) 71–80.
  23. Oladipo, I. C., Lateef, A., Azeez, M. A., Asafa, T. B., Yekeen, T. A., Ogunsona, S. B., Irshad, H. M., Abbas, S. H., “Antidiabetic properties of phytosynthesized gold nanoparticles (Aunps) from Datura stramonium seed, IOP Conference Series Materials Science and Engineering, 805 (2020) 012035.
  24. Lateef, A., Ojo, S. A., Elegbede, J. A., Akinola, P. O., Akanni, E. O., “Nanomedical applications of nanoparticles for blood coagulation disorders”, Environmental Nanotechnology, Springer International Publishing, 1 (2018) 243–277.
  25. Gomathi, A. C., Xavier Rajarathinam, S. R., Mohammed Sadiq, A., Rajeshkumar, S., “Anticancer activity of silver nanoparticles synthesized using aqueous fruit shell extract of Tamarindus indica on MCF-7 human breast cancer cell line”, Journal of Drug Delivery Science and Technology, 55 (2020) 101376.
  26. Tessy, J., Kokila, A., Parmar., Shailesh, C., Kotval., Jayesh, J., “Synthesis, Characterization, Antibacterial and Anticancer Properties of Silver Nanoparticles Synthesized from Carica Papaya Peel Extract”, J. Nanosci. Nanotechnol, 17(1) (2021) 23-32.
  27. Kurek, A., Grudniak, A. M., Kraczkiewicz-Dowjat, A., Wolska, K. I., “New antibacterial therapeutics and strategies”, J. Microbiol, 60(1) (2011) 3-12.
  28. Mathew, T. V., Kuriakose, S., “Studies on the antimicrobial properties of colloidal silver nanoparticles stabilized by bovine serum albumin”, Colloids Surf. B. Biointerfaces, 101 (2013)14-18.
  29. Vijayaraghavan, K., Nalini, S. P. K., Prakash, N. U., Madhankumar, D., “One step green synthesis of silver nano/microparticles using extracts of Trachyspermum ammiand Papaver somniferum”, Colloids Surf. B. Biointerfaces, 94 (2012) 114–117.
  30. Daniel, S. C. G. K., Kumar, R., Sathish, V., Sivakumar, M., Sunitha, S., Sironmani, T. A., “Green Synthesis (Ocimum tenuiflorum) of Silver Nanoparticles and Toxicity Studies in Zebra Fish (Danio rerio)”, J. Nanosci. Nanotechnol, 2(2) (2011) 103–117.
  31. Sulochana, S., Krishnamoorthy, P., Sivaranjani, K., “Synthesis of Silver Nanoparticles using Leaf Extract ofAndrographis paniculata”, Pharmcol. Toxico, 7(5) (2012) 251–258.
  32. Ghaffari-Moghaddam, M., Hadi-Dabanlou, R., “Plant mediated green synthesis and antibacterial activity of silver nanoparticles usingCrataegus douglasii fruit extract” Ind. Eng. Chem, 20 (2014) 739–744.
  33. Roopan, S. M., Rohit, G. M., Madhumitha, G., Rahuman, A. A., Kamaraj, C., Bharathi, A., Surendra, T., “Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity”, Crop Prod, 43 (2013) 631–635.
  34. Saxena, A., Tripathi, R. M., Singh, R. P., “Biological synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity”, J. Nanomater. Bios, 5(2) (2010) 427–432.
  35. Sukumar, S., Rudrasenan, A., Padmanabhan Nambiar, D., “Green-synthesized rice-shaped copper oxide nanoparticles using Caesalpinia bonducella seed extract and their applications”, ACS Omega, 5(2) (2020) 1040–1051.
  36. Murthy, H. C. A., Desalegn, T., Kassa, M., Abebe, B., Assefa, T., “Synthesis of green copper nanoparticles using medicinal plant Hagenia abyssinica (Brace) jf. Gmel. leaf extract: Antimicrobial properties”, Journal of Nanomaterials, (2020) 1–12.
  37. Javed, B., Nadhman, A., Mashwani, Z. R., “Phytosynthesis of Ag nanoparticles from Mentha longifolia: Their structural evaluation and therapeutic potential against HCT116 colon cancer, Leishmanial and bacterial cells”, Applied Nanoscience, 10(9) (2020) 3503–3515.
  38. Paseban, N., Ghadam, P., Purohosseini, P. S., “The Fluorscence Behavior and Stability of AgNP Synthesized by Juglan Regia Green Husk Aqueou Extract”. J. Nanosci. Nanotechnol, 15 (2019) 117-126.
  39. Nzekekwu, A. K., Abosede, O. O., “Green synthesis and characterization of silver nanoparticles using leaves extracts of neem (Azadirachta indica) and bitter leaf (Vernonia amygdalina)”, Journal of Applied Sciences and Environmental Management, 23(4) (2019) 695–699.
  40. Ghotekar, S., Pansambal, S., Pawar, S. P., Pagar, T., Oza, R., Bangale, S., “Biological activities of biogenically synthesized fluorescent silver nanoparticles using Acanthospermum hispidum leaves extract”, SN Applied Sciences, 1(11) (2019) 1342.
  41. Qayyum, S., Oves, M., Khan, A. U., “Obliteration of bacterial growth and biofilm through ROS generation by facilely synthesized green silver nanoparticles”, PLOS ONE, 12(8) (2017) e0181363.
  42. Fatema S., Shirsat M., Farooqui M., Arif P. M., “Biosynthesis of silver nanoparticle using aqueous extract of Saraca asoca leaves, its characterization and antimicrobial activity”, J. Nano Dimens, 10 (2019) 163-168.
  43. Azarbani, F., Shiravand, S., “Green synthesis of silver nanoparticles by Ferulago macrocarpa flowers extract and their antibacterial, antifungal and toxic effects”, Green Chemistry Letters and Reviews, 13(1) (2020) 41–49.
  44. Vala, A. K., Shah, S., “Rapid synthesis of silver nanoparticles by a marine-derived fungus Aspergillus Niger and their antimicrobial potentials”, J. Nanosci. Nanotechnol, 8 (2012) 197-206.
  45. Jha, A. K., Prasad, K., Kulkarni, A. R., “Yeast mediated synthesis of silver nanoparticles”, J. Nanosci. Nanotechnol, 4 (2008) 17-22.
  46. Chandrasekhara Rao, T., Lakshminarayana, G., Prasad, N. B. L., Sagan Mohan Rao, S., Azeemoddin, G., Atchynta Ramayya, D., Thirumala Rao, S. D., “Characteristics and compositions of Carissa spinarum,Leucaena leucocephala and Physalis minima seeds and oils”, Am. Oil. Chem. Soc, 61(9) (1984) 1472-1473.
  47. Gamal-Eldeen, A.M., Amer, H., Helmy, W. A., Ragab, H. M., Talaat, R. M., “Antiproliferative and cancer-chemopreventive properties of sulfated glycosylated extract derived from Leucaena leucocephala”, Indian J. Pharm. Sci, 69(6) (‎2007) 805-811.
  48. Deodhar, U. P., Paradkar, A. R., Purohit, A. P., “Preliminary evaluation of Leucaena leucocephala seed gum as a tablet binder”, Dev. Ind. Pharm, 24(6) (1998) 577-582.
  49. Verma, P. R. P., Balkishen, R., “Studies on disintegrant action of Leucaena leucocephala seed gum in ibuprofen tablet and its mechanism”, Sci. Ind. Res, 66, (2007) 550-557.
  50. Irene, M. V., Robert, M. T. G., Rosette, C. G., “Bioactivity studies on the alkaloid extracts from seeds of Leucaena leucocephala”, Res, 11(8) (1997) 615–617.
  51. Ademola, I. O., Akanbi, A. I., Idowu, S. O., “Anthelmintic activity of Leucaena leucocephala chromatographic seed fractions on gastrointestinal sheep nematodes”, Biol, 45(7) (2005) 599-604.
  52. Shrivastava, V. S., “Removal of Congo red dye from aqueous solution by Leucaena leucocephala (Subabul) seed pods” International Journal of Chem Tech Research, 4(3) (2012) 1038-1043.
  53. Gutteridge, C., Shelton, H. M., “Forage Tree Legumes in Tropical Agriculture” Tropical Grassland Society of Australia, Australia (1998).
  54. Selvaraj, R., Ramesh, V., Varadavenkatesan, T., “Green biosynthesis of silver nanoparticles using Calliandra haematocephala leaf extract, their antibacterial activity and hydrogen peroxide sensing capability” J. Chem, 10(2) (2015) 253-261.
  55. Rakholiya, K., Chanda, S., “In vitro interaction of certain antimicrobial agents in combination with plant extracts against some pathogenic bacterial strains”, Asian Pac. J. Trop. Biomed, 2 (2012) 876–880.
  56. Venkatesham, M., Ayodhya, D., Madhusudhan, A., Santoshumari, A., Veerabhadram, G., Girija Mangatayaru, K., “A Novel Green Synthesis of Silver Nanoparticles Using Gum Karaya: Characterization, Antimicrobial and Catalytic Activity Studies”, Clust. Sci, 25(2) (2014) 409–422.
  57. Jegadeeswaran, P., Rajiv, P., Rajeshwari Shivaraj, P., Venkatesh, R., “Photo catalytic degradation of dye using brown seaweed mediated silver nanoparticles”, Bio. sci, 3(4) (2012) 229-233.
  58. Zhang, W., Qiao, X., Chen, J., “Synthesis and characterization of silver nanoparticles in AOT microemulsion system”, Phy, 330(3) (2006) 495–500.
  59. Parashar, U. K., Saxenaa, P., Srivastava, A., “Bioinspired synthesis of silver nanoparticles”, J. Nanomater. Biostruct, 4(1) (2009) 159–166.
  60. Govindaraju, K., Tamilselvan, S., Kiruthogs, V., Simgaravelu, G., “Biogenic silver nanoparicles by Solanum torvum and their promising antimicrobial activity”, Biopest. 3 (2010) 394–399.
  61. Raut, R. W., Kolekar, N. S., Lakkakula, J. R., Mendhulkar, V. D., Kashid, S. B., “Photosynthesis of silver nanoparticles using Gliricidia sepium (Jecq)”, Nanosci, 5 (2009) 117–122.
  62. Ramchandra Nalwade, A., Swati Sudhir, S., Gajanan Laxman, B., Namdeo Bhagwan, A., Sambhaji Dagadu, S., Vaishali Vasant, G., “Rapid biosynthesis of silver nanoparticles using bottle gourd fruit extract and potential application as bactericide”, Research in Pharmacy, 3(3) (2013) 22-28.
  63. Nestor, A. R. V., Mendieta, V. S., Lopez, M. A. C., Espinosa, R. M. G., Lopez, M. A. C., Alatorre, J. A. A., “Solventless synthesis and optical properties of Au and Ag nanoparticles using Camiellia sinensis extract,” Lett, 62(17) (2008) 3103–3105.
  64. Nagajyothi, P. C., Sreekanth, T. V. M., Lee, J., Lee, K. P., “Mycosynthesis: Antibacterial, antioxidant and antiproliferative activities of silver nanoparticles synthesized from Inonotus obliquus (Chaga mushroom) extract”, Photochem. Photobiol. B, 130 (2014) 299–304.
  65. Huang, L., Zhai, M., Peng, J., Xu, L., Li, J., Wei, G. J., “A fluorescence quenching method for determination of copper ions with CdTe quantum dots”, Colloid Interface Sci, 316 (2007) 398–404.
  66. Mat Yusuf, S. N. A., Che Mood, C. N. A., Ahmad, N. H., Sandai, D., Lee, C. K., Lim, V., “Optimization of biogenic synthesis of silver nanoparticles from flavonoid-rich Clinacanthus nutans leaf and stem aqueous extracts”, Royal Society Open Science, 7(7) (2020) 200065.
  67. Ramya juliet, M., “Biogenic synthesis of copper nanoparticles using aquatic pteridophyte Marsilea quadrifolia Rhizome and its antibacterial activity”, Int J Nano Dimens, 11 (2020) 337–345.
  68. Ghotekar, S., Savale, A., Pansambal, S., “Phytofabrication of fluorescent silver nanoparticles from Leucaena leucocephala leaves and their biological activities”, Journal of Water and Environmental Nanotechnology, 3(2) (2018) 95-105.
  69. Songa, J. Y., Janga, H. K., Kim, B. S., “Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts”, Process Biochem, 44 (2009) 1133–1138.
  70. Eya’ane Meva, F., Segnou, M. L., Okalla Ebongue, C., Ntoumba, A. A., Belle Ebanda Kedi, P., Deli, V., Etoh, M. A., Mpondo Mpondo, E., “Spectroscopic synthetic optimizations monitoring of silver nanoparticles formation from Megaphrynium macrostachyum leaf extract”, Revista Brasileira de Farmacognosia, 26(5) (2016) 640–646.
  71. Awwad, A. K. L. M., Salem, N. M., Abdeen, A., “Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity”, J. Ind. Chem, 4 (2013) 29-34.
  72. Awwad, A. M., Salem, N. M., Ibrahim, Q. M., Abdeen, A. O., “Phytochemical fabri-cation and characterization of silver/silver chloride nanoparticles using Albizia julibrissin flowers extract”, Matter Lett, 6 (2015) 726–730.
  73. Peron, S., Hadi, F., Azarbani, F., Ananda Murthy, H. C., “Antimicrobial, antioxidant, anti-glycation and toxicity studies on silver nanoparticles synthesized using Rosa damascena flower extract”, Green Chemistry Letters and Reviews, 14(3) (2021) 519–533.
  74. Kaviya, S., Santhanalakshmi, J., Viswanathan, B., Muthumary, J., Srinivasan, K., “Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity”, Acta. A. Mol. Biomol. Spectrosc, 79 (2011) 594–598.
  75. Raut, R. W., Kolekar, N. S., Lakkakula, J. R., Mendhulkar, V. D., Kashid, S. B., “Extracellular synthesis of silver nanoparticles using dried leaves of Pongamia pinnata (L) pierre”, Nano-Micro Letters, 2(2) (2010) 106–113.
  76. Muthuvel, A., Jothibas, M., Manoharan, C., “Synthesis of copper oxide nanoparticles by chemical and biogenic methods: Photocatalytic degradation and in vitro antioxidant activity”, Nanotechnology for Environmental Engineering, 5(2) (2020) 14.
  77. Abebe, B., Zereffa, E. A., Tadesse, A., Murthy, H. C. A., “A review on enhancing the antibacterial activity of ZNO: Mechanisms and microscopic investigation”, Nanoscale Research Letters, 15(1) (2020) 190.
  78. Biswal, A. K., Misra, P. K., “Biosynthesis and characterization of silver nanoparticles for prospective application in food packaging and biomedical fields”, Materials Chemistry and Physics, 250 (2020) 123014.
  79. Yawadio Nsimba, R., Kikuzaki, H., Konishi, Y., “Antioxidant activity of various extracts and fractions of Chenopodium quinoa and Amaranthus spp. Seeds”, Food Chemistry, 106(2) (2008) 760–766.
  80. Rajesh, R., Kumar, S. S., Venkatesan, R., “Efficient degradation of azo dyes using Ag and Au nanoparticles stabilized on graphene oxide functionalized with PAMAM dendrimers”, New J. Chem, 38 (2014) 1551–1558.
International Journal of Nanoscience and Nanotechnology
Volume 18, Issue 1
March 2022
Pages 65-78

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