Synthesis, Characterization, and In Vitro Drug Release and In Vitro Antibacterial Activity of O/W Nanoemulsions Loaded with Natural Eucalyptus Globulus Essential Oil
1
PG Dept of Biotechnology, Lyallpur Khalsa College, Jalandhar, Punjab, India
2
Dept of Physics, Lyallpur Khalsa College, Jalandhar, Punjab, India
Abstract
Due to diverse biological activities, eucalyptus oil from eucalyptus waste leaves has tremendous applications in food and pharmaceutical industries. Since it is highly unable, hydrophobic and volatile and so easily loses its anti-microbial activity. To protect this herbal product having bioactive properties, nanoemulsion technology is the best way to encapsulate it. The objective of this study was synthesis of O/W type nanoemulsions, its characterization and evaluation of biological activities. O/W type eucalyptus oil based nanoemulsions were prepared by using sodium dodecyl sulphate, tween 20. Other physiochemical parameters viz; conductivity, pH, and antibacterial activity were also studied. UV, fluorescent and FT-IR techniques were also used. Drug release and pharmokinetic studies were also conducted. Stability of nanoemulsions was studied for 50 days. As compared to tween 20, SDS based nanoemulsions were smaller is size. Nanoparticles were spherical in shape and maximum encapsulation efficiency of 58%. Nanoparticles were stable even after 50 days of storage. Both nanoemulsions exhibited antibacterial and anti-inflammatory activities. Pharmokinetic studies revealed that formulated nanoemulsions followed korsmeyer-Peppas model. All these observations indicated that nanoemulsions from eucalyptus oil have key potential to act as key therapeutic agents in pharmaceutical and food based industries.
Raho, G. B., Benali, M., “Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus”,Asian Pac J Trop Biomed, 2 (2012) 739-42 .
Goodger, J. Q. D., Senaratne, S. L., Nicolle, D., Woodrow, I. E., “Correction: Foliar Essential Oil Glands of Eucalyptus Subgenus Eucalyptus (Myrtaceae) are a rich source of flavonoids and related non-volatile constituents”, PLOS ONE, 11 (2016): e0155568.
Moghimi, , Ghaderi, L., Rafati, H., Aliahmadi, A., McClements, D. J., “Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against E. coli”, Food Chem, 194 (2016) 410-415.
Qian, C., Decker, E. A., Xiao, , McClements, D. J., “Nanoemulsion delivery systems: Influence of carrier oil on β-carotene bioaccessibility”, Food Chem, 135 (2012) 1440-7
Al-Edresi, S., Baie, S., “Formulation and stability of whitening VCO-in-water nano-cream”, Int J Pharmaceutics, 373 (2009)174-178.
Kerwin, B. A., “Polysorbates 20 and 80 used in the formulation of protein bio therapeutics: structure and degradation pathways,” Pharm. Sci, 97 (2008) 2924-2935.
Bohrey, S., Chourasiya, V., Pandey, A., “Polymeric nanoparticles containing diazepam: preparation, optimization, characterization, in-vitro drug release and release kinetic study”, Nano Convergence, 3 (2016) 31-38.
Sharma, A. D., Kaur, I., Singh,, “Tryptophan fluorescence spectroscopy: key tool to study protein denaturation/anti-inflammatory assay”, Res Rev Biotechnol Biosci, 8 (2021) 90-94.
Moradi, S., Barati, A., “Essential Oils Nanoemulsions: Preparation, Characterization and Study of Antibacterial Activity against Escherichia Coli”, Int J Nanosci Nanotechnol, 15 (2019)199-210.
Silva, H. D., Cerqueira, M. A., Vicente, A. A., “Influence of surfactant and processing conditions in the stability of oil-in-water nanoemulsions”, J Food Eng, 167 (2015) 89-98.
Talegaonkar, S., Tariq, M., Alabood, R. M., “Design and Developement of O/W Nanoemulsion for the Transdermal Delivery of Ondansetron”, Bull Pharm Res, 1 (2011) 18-30.
Kumar, V., Singh, S., Singh, A., Dixit, A. K., Shrivastava, S., Kondalkar, S. A., Subhose, V., “Determination of phytochemical, antioxidant, antimicrobial, and protein binding qualities of hydroethanolic extract of Celastrus paniculatus”, Applied Biol. Biotechnol., 6 (2018) 11-17.
Tomaszewsk, E., Soliwod, K., Kadziol, K., Tkacz-Szczesn, B., Celichowski, G., Cichomski, M., Szmaj, W., Grobelny, J., “Detection Limits of DLS and UV-Vis Spectroscopy in Characterization of Polydisperse Nanoparticles Colloids”, Nanomaterials, 1 (2013) 10 .
Sharma, A. D., Farmaha, M., Kaur, I., Singh, N, “Phytochemical analysis using GC-FID, FPLC fingerprinting, antioxidant, antimicrobial, anti- inflammatory activities analysis of traditionally used Eucalyptus globulus essential oil”, Drug Ana Res, 5 (2021) 26-38.
Boni, M., Nastasa, V., Andrei, I. R., Staicu, A., Pascu, M. L., “Enhanced fluorescence emitted by microdroplets containing organic dye emulsions”, Biomicrofluidics , 24 (2015) 9(1) 014126.
Mylle, E., Codreanu, C., Boruc, J., Russinova, E., “Emission spectra profiling of fluorescent proteins in living plant cells”, Plant Methods, 9 (2013) 1–8.
Chen, J. B., Sun, S. Q., Tang, X. D., Zhang, J. Z., Zhou, Q., “Direct and simultaneous detection of organic and inorganic ingredients in herbal powder preparations by Fourier transform infrared microspectroscopic imaging”, Acta A, 165 (2016)176–182.
Paulraj, , Irudayaraj, V., Johnson, M., Patric, D., “Phytochemical and anti-bacterial activity of epidermal glands extract of Christella parasitica (L.) H. Lev”, Asian Pac J Trop Biomed, 1 (2011) 8-11.
Talamond, P., Verdeil, J. L., Conejero, , “ Secondary metabolite localization by auto-fluorescence in living plant cells”, Molecules , 20 (2015) 5024-5037.
Swamy, M. K., Akhtar, M. S., Sinniah, U. R., “Antimicrobial Properties of Plant Essential Oils against Human Pathogens and Their Mode of Action: An Updated Review", Evidence-Based Complementary Alternative Medicine, Article ID 3012462, 21 (2016).
Sharma, A. D., Kaur, I., & Singh, N. (2021). Synthesis, Characterization, and In Vitro Drug Release and In Vitro Antibacterial Activity of O/W Nanoemulsions Loaded with Natural Eucalyptus Globulus Essential Oil. International Journal of Nanoscience and Nanotechnology, 17(3), 191-207.
MLA
A. D. Sharma; I. Kaur; N. Singh. "Synthesis, Characterization, and In Vitro Drug Release and In Vitro Antibacterial Activity of O/W Nanoemulsions Loaded with Natural Eucalyptus Globulus Essential Oil". International Journal of Nanoscience and Nanotechnology, 17, 3, 2021, 191-207.
HARVARD
Sharma, A. D., Kaur, I., Singh, N. (2021). 'Synthesis, Characterization, and In Vitro Drug Release and In Vitro Antibacterial Activity of O/W Nanoemulsions Loaded with Natural Eucalyptus Globulus Essential Oil', International Journal of Nanoscience and Nanotechnology, 17(3), pp. 191-207.
VANCOUVER
Sharma, A. D., Kaur, I., Singh, N. Synthesis, Characterization, and In Vitro Drug Release and In Vitro Antibacterial Activity of O/W Nanoemulsions Loaded with Natural Eucalyptus Globulus Essential Oil. International Journal of Nanoscience and Nanotechnology, 2021; 17(3): 191-207.
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