Biogenic Approach using Sheep Milk for the Synthesis of Platinum Nanoparticles: The Role of Milk Protein in Platinum Reduction and Stabilization

Document Type: Research Paper


1 Department of Mycology, Pasteur Institute of Iran, Tehran 13164, Iran

2 Faculty of Aerospace, Science and Research Campus, Islamic Azad University, Tehran, Iran

3 Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115-331, Iran

4 Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran 13164, Iran

5 Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

6 Department of Mycology, Pasteur Institute of Iran


Bio-directed synthesis of nanoparticles is an interesting field of rapid advancement for biologists,
chemists and materials scientists, especially in light of efforts to find out green methods of inorganic
material synthesis. In the present study, green synthesis of platinum nanoparticles (PtNPs) using sheep
milk is reported for the first time. By adjusting the concentrations of chloroplatinic acid (H
2PtCl6) and
milk in aqueous solutions, spherical PtNPs were obtained at room temperature. The nanoparticles
obtained were characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), high-resolution
Transmission Electron Microscopy (TEM) and X-Ray diffraction (XRD). The spherical particles obtained
have an average size 9.0 nm as shown by XRD pattern and TEM analysis. Fourier Transform Infra-Red
(FTIR) measurements were carried out to identify the possible biomolecules responsible for capping and
efficient stabilization of the PtNPs synthesized by milk. The carboxylic acid group libration and amide I
and II bands revealed the binding of protein with platinum surface through the amine group rather than
the carboxyl group
. Among a wide variety of biological resources which are introduced for reducing Pt
ions into PtNPs, green approaches like using sheep milk have outstanding benefits for synthesize
biological metal nanoparticles due to their eco-friendly phenomenon and very less amounts of
cytotoxicity. PtNPs green synthesized in this study are potential candidates to use in drug discovery and
gene transfer technologies.