International Journal of Nanoscience and Nanotechnology

International Journal of Nanoscience and Nanotechnology

Green synthesis and characterization of bioinspired metal nanoparticles mediated by Ipomoea involucrata P. Beauv. as potential antimicrobial agents

Document Type : Research Paper

Authors
Ikechukwu P. Ejidike 1
Doris O. Seyinde 1
Chidera F. Nwaeme 1
Mercy O. Bamigboye 2
Olajumoke Oluade 3
Racheal U. Ijimdiya 1
Temitope O. Fakoya 4
Dorcas A. Fadare 1
Oluwatoyin O. Ojo 1
Solomon A. Olaleru 5
Juliana B. Adetunji 6
Hadley S. Clayton 7
1 Department of Chemical Sciences, Faculty of Science, Anchor University, Lagos, Nigeria
2 Department of Industrial Chemistry, Faculty of Physical Sciences, University of Ilorin, Ilorin, Nigeria
3 Department of Chemistry, Faculty of Science, Gombe State University, Gombe, Nigeria
4 Department of Industrial Chemistry, Faculty of Science, University of Ilesa, Ilesa, Nigeria
5 Department of Physical Science, School of Science, Yaba College of Technology, Lagos, Nigeria
6 Department of Biochemistry, Faculty of Basic and Applied Sciences, Osun State University, Oshogbo, Nigeria
7 Department of Chemistry, College of Science, Engineering, and Technology, University of South Africa, Florida Park, South Africa
10.22034/ijnn.2025.2010480.2422
Abstract
Chemical, physical, or biological routes have been beneficial to the synthesis of nanoparticles of varied shapes and sizes. Nevertheless, the synthesis of nanoparticles via the use of plants as precursors is a rapid, environmentally friendly, low-cost option and is safe for the human populace. Synthesis of metal nanoparticles with plant extracts is advantageous owing to their ease of scalability and capability as a capping, bio-reducing mediator, and/ or stabilizer. This study presents a simple and eco-friendly approach for the synthesis of AgNPs, MnNPs, NiNPs, and CuNPs from the leaf extract of Ipomoea involucrata. The synthesized nanoparticles were characterized by UV-UV-visible spectroscopy, Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDX), and Fourier Transform Spectroscopy (FTIR). UV-vis spectra of the aqueous medium containing the metal nanoparticles showed a peak at 425 nm for AgNPs, CuNPs at 433 nm, and NiNPs around 416 nm, while MnNPs showed a peak at about 417 nm. The morphology of the metal nanoparticles (IP-MNPs) as documented by the SEM results reveals irregular shapes as peculiar to each nanoparticle. The EDX analysis confirms the presence of each elemental metal in the synthesized nanoparticles. Furthermore, the nanoparticles showed better activities against microbial pathogens than the plant extract; it is therefore suggested that these green-synthesized metal nanoparticles tagged with Ipomoea involucrata can address future health and medical concerns
Keywords
Green synthesis
Ipomoea involucrata
Nanoparticles
Antimicrobial
Spectroscopy

Subjects

  1. Gobinath, E., Dhatchinamoorthy, M., Saran, P., Vishnu, D., Indumathy, R., Kalaiarasi, G., “Synthesis and characterization of NiO nanoparticles using Sesbania grandiflora flower to evaluate cytotoxicity”, Results Chem., 6 (2023) 101043.
  2. Ettadili, F. E., Aghris, S., Laghrib, F., Farahi, A., Saqrane, S., Bakasse, M., Lahrich, S., El Mhammedi, M.A., “Recent advances in the nanoparticles synthesis using plant extract: Applications and future recommendations”, J. Mol. Struct., 1248 (2021) 131538.
  3. Montazersaheb, S., Eftekhari, A., Shafaroodi, A., Tavakoli, S., Jafari, S., Baran, A., Baran, M. F., Jafari, S., Ahmadian, E., “Green-synthesized silver nanoparticles from peel extract of pumpkin as a potent radiosensitizer against triple-negative breast cancer (TNBC)”, Cancer Nano 15 (2024) 47.
  4. Mathur, P., Jha, S., Ramteke, S., Jain, N. K., “Pharmaceutical aspects of silver nanoparticles”, Artif Cells Nanomed. Biotechnol., 46 (2018) 115–126
  5. Ejidike, I. P., Ogunleye, O., Bamigboye, M. O., Ejidike, O. M., Ata, A., Eze, M. O., Clayton, H. S., Nwankwo, V. U., Fatokun, J. O., “Role of nanotechnology in medicine: Opportunities and challenges”, In: Shah, M.P., Bharadvaja, N., Kumar L. (eds). Biogenic Nanomaterials for Environmental Sustainability: Principles, Practices, and Opportunities. Environmental Science and Engineering. Springer, Cham Pg., (2024) 353-375.
  6. Shah, S., Shah, S. A., Faisal, S., Khan, A. A., Ullah, R., Ali, N., Bilal, M., “Engineering novel gold nanoparticles using Sageretia thea leaf extract and evaluation of their biological activities”, J. Nanostructure Chem., 12 (2021) 129-140.

 

 

 

 

  1. Ullah, R., Shah, S., Muhammad, Z., Shah, S., Faisal, S., Khattak, U., Haq, T., Taj-Akbar, M., “In vitro and in vivo applications of Euphorbia wallichii shoot extract mediated gold nanospheres”, Green Process. Synth., 10 (2021) 101-111.
  2. Ogunsile, B. O., Okoh, O. S., Ejidike, I. P., Omolaja, O. R. “Biosynthesis and optimization of AgNPs yield from Chromolaena Odorata leaf extract using response surface methodology (RSM)”, Phys. Chem. Res., 12 (2024) 21-31.
  3. Do, H. T. T., Nguyen, N. P. U., Saeed, S. I., Dang, N. T., Nguyen, T. T. H., “Advances in silver nanoparticles: unraveling biological activities, mechanisms of action, and toxicity”. Appl. Nanosci., 15 (2025) 1.
  4. Ejidike, I. P., Bamigboye, O. M., Ijimdiya, R. U., Seyinde, D. O., Ojo, O. O., “Synthesis, characterization and biological evaluation of hexagonal wurtzite structured ZnO nanoparticle from Zn(II)-Schiff base complex”, Proceed. Niger. Acad. Sci., 13 (2020) 136-147.
  5. Haleem, A., Javaid, M., Singh, R. P., Rab, S., Suman, R., “Applications of nanotechnology in medical field: a brief review”, Glob. Health J., 7 (2023) 70-77.
  6. Ejidike, I. P., Direm, A., Parlak, C., Bamigboye, M. O., Oluade, O., Adetunji, J. B., Ata, A., Eze, M. O., Hollett, J. W., Clayton, H. S., “Cadmium oxide nanoparticles from new organometallic Cd(II)-Schiff base complex and in vitro biological potentials: Dual S. aureus and E. coli DNA gyrase inhibition by the precursors via in-silico binding modes’ study”, Res. on Chem. Intermed., 50 (2024) 2763–2791.
  7. Keskin, C., Ölçekçi, A., Baran, A., Baran, M. F., Eftekhari, A., Omarova, S., Khalilov, R., Aliyev, E., Sufianov, A., Beilerli, A., Gareev, I., “Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities”, Front. Chem., 11 (2023) 1187808.
  8. Adamu, S., Pindiga, N. Y., Nuhu, A. H., Ibrahim, A., Yakubu, M. S., “Green synthesis of copper and iron nanoparticles from extracts of Eucalyptus with their antimicrobial activities”, Science World Journal, 19 (2024) 279-283.
  9. Ejidike, I. P., Clayton, H. S., “Green synthesis of silver nanoparticles mediated by Daucus carota L.: antiradical, antimicrobial potentials, in vitro cytotoxicity against brain glioblastoma cells”, Green Chem. Lett. Rev., 15 (2022) 297-310.
  10. Olaleru, S. A., Molokwu, M. I., Mathew, S., Ejidike, I. P., Oyebamiji, O. O., “Enhanced photocatalytic degradation of methylene blue dye using TiO2 nanoparticles obtained via chemical and green synthesis: a comparative analysis”, Pure Appl. Chem., 97 (5) (2025). https://doi.org/10.1515/pac-2024-0326
  11. Emeribe, J., Nkwoada, A., Bilar, A., Alisa, C., “Green synthesis of silver nanoparticle using Okoubaka aubrevillei for antimicrobial treatment”, Int. J. Nanosci. Nanotechnol., 20 (3) (2024) 219-229.
  12. Abdelghany, T. M, Al-Rajhi, A. M. H., Al Abboud, M. A., Alawlaqi, M. M., Magdah, A. G., Helmy, E. A. M., Mabrouk, A. S., “Recent advances in green synthesis of silver nanoparticles and their applications: About future directions, A review”, BioNanoSci., 8 (2018) 5–16.
  13. Nishanthi, R., Malathi, S., Paul-S., J., Palani, P., “Green synthesis and characterization of bioinspired silver, gold and platinum nanoparticles and evaluation of their synergistic antibacterial activity after combining with different classes of antibiotics”, Mater. Sci. Eng.: C Mater. for Biol. Appl., 96 (2019) 693-707.
  14. Namburi, K. R., Kora, A. J., Chetukuri, A., Kota, V. S. M. K., “Biogenic silver nanoparticles as an antibacterial agent against bacterial leaf blight causing rice phytopathogen Xanthomonas oryzae pv. Oryzae”, Bioprocess Biosys. Eng., 44 (2021) 1975–1988.
  15. Al-Rajhi, A. M. H., Salem, S. S., Alharbi, A. A., Abdelghany, T. M., “Ecofriendly synthesis of silver nanoparticles using Kei-apple (Dovyalis caffra) fruit and their efficacy against cancer cells and clinical pathogenic microorganisms”, Arab. J. Chem., 15 (2022) 103927.
  16. Ejidike, I. P., Ijimdiya, R. U., Emmanuel-Akerele, H. A., Emmanuel, G. C., Ejidike, O. M., Bamigboye, M. O., Seyinde D. O., Olaleru, A., Tanimowo, W. O., Awolope, R. O., “Biosynthesis, characterization, and antimicrobial assessment of metal nanoparticles from Dryopteris manniana (HOOK.) C. Chr leaf extract”, Bull. Pharm. Sci. Assiut Univ., 46 (2023) 225-238.
  17. Bamigboye, O. M., Ejidike, I. P., Lawal, M., “Synthesis, characterization, and antimicrobial potentials of some flavonoid-metal complexes from Chromolaena Odorata”, Iraqi J. Sci., 61 (2020) 2440-2447.
  18. Ejidike, I. P., Fadare, D. A., Bamigboye, O. M., Ijimdiya, R. U., Lasore, I. C., Oladokun, M. S., Olajumoke, O., “Phytochemical constituents of biological prominence from medicinal plants as therapeutic agents in disease treatment”, Anchor University Journal of Science and Technology, 2 (2022) 89-114.
  19. Hamilton-Amachree, A., Fadare, D. A., Uzoekwe, N. M., Ejidike, I. P., “Phytochemicals, nutritional and antibacterial evaluation of a domestic plant - Tetradenia riparia”, Al-Qadisiyah Journal of Pure Science, 29 (2024) 94-98.
  20. Dimo, S. N., Obidi, O. F., Nejo, A. O., Olaleru, S. A., Ejidike, I. P., Adetona, A. J., “Biofabrication, spectroscopic, and photocatalytic studies of titania nanoparticles mediated by Proteus mirabilis strain NG-ABK-32 for smart applications”, Smart Sci., 12 (2024) 373–386.
  21. Vorobyova, V., Vasyliev, G., Uschapovskiy, D., Lyudmyla, K., Skiba, M., “Green synthesis, characterization of silver nanoparticals for biomedical application and environmental remediation”, J. Microbio. Methods, 193 (2022) 106384.
  22. Dimo, S. N., Obidi, O. F., Nejo, A. O., Olaleru, S. A., Ejidike, I. P., Adetona, A. J., “Preparation of nanosized TiO2 in the presence of bacteria Bacillus paralicheniformis and its photocatalytic properties in the process of decolorization of dyes”, Theor. Exp. Chem., 60 (2024) 194-201.
  23. Osuntokun, J., Onwudiwe, D. C., Ebenso, E. E., “Green synthesis of ZnO nanoparticles using aqueous Brassica oleracea L. var. italica and the photocatalytic activity”. Green Chem. Lett. Rev., 12 (2019) 444–457.
  24. Beegum, S. A., David, S. B., “Investigation of antimicrobial activity of ‎plant-mediated green synthesis of silver ‎nanoparticles”, Int. J. Nanosci. Nanotechnol., 18 (4) (2022) 265-274.
  25. Mosaviniya, M., Kikhavani, T., Tanzifi, M., Yaraki, M. T., Tajbakhsh, P., Lajevardi, A., “Facile green synthesis of silver nanoparticles using Crocus Haussknechtii bois bulb extract: Catalytic activity and antibacterial properties”, Colloids Interface Sci. Commun., 33 (2019) 100211.
  26. Andleeb, A., Andleeb, A., Asghar, S., Zaman, G., Tariq, M., Mehmood, A., Nadeem, M., Hano, C., Lorenzo, J. M., Abbasi, B. H., “A systematic review of biosynthesized metallic nanoparticles as a promising anti-cancer-strategy”, Cancers (Basel), 13 (2021) 2818.
  27. Bruna, T., Maldonado-Bravo, F., Jara, P., Caro, N.,“Silver nanoparticles and their antibacterial applications”, Int. J. Mol. Sci., 22 (2021) 7202.
  28. Lail, N. U., Sattar, A., Omer, M. O., Hafeez, M. A., Khalid, A.R., Mahmood, S., Shabbir, M. A., Ahmed, W., Aleem, M. T., Alouffi, A., Almutairi, M. M., “Biosynthesis and characterization of zinc oxide nanoparticles using Nigella sativa against coccidiosis in commercial poultry”, Sci. Rep., 13 (2023) 6568.
  29. Lekshmi, N. C., Sumi, S. B., Viveka, S., Jeeva, S., Brindha, J. R., “Antibacterial activity of nanoparticles from Allium sp”, J. Microbio. Biotech. Res., 2 (2012) 115-119.
  30. Dinga, E., Mthiyane, D. M. N., Upenyu Marume, U., Botha, T. L., Horn, S., Pieters, R., Wepener, V., Ekennia, A., Onwudiwe, D. C., “Biosynthesis of ZnO nanoparticles using Melia azedarach seed extract: Evaluation of the cytotoxic and antimicrobial potency”, OpenNano, 8 (2022) 100068.
  31. Murthy, H. C., Desalegn, T., Kassa, M., Abebe, B., Assefa, T., “Synthesis of green copper nanoparticles using medicinal plant Hagenia abyssinica (Brace) JF. Gmel. leaf extract: Antimicrobial properties”, J. Nanomater., 2020 (2020) 3924081.
  32. Jaison, J. P., Balasubramanian, B., Gangwar, J., James, N., Pappuswamy, M., Anand, A. V., Al-Dhabi, N. A., Valan Arasu, M., Liu, W. -C., Sebastian, J. K., “Green synthesis of bioinspired nanoparticles mediated from plant extracts of Asteraceae family for potential biological applications”, Antibiotics, 12 (2023) 543.
  33. Essiet, U. A., Ukpong, U. J., “Comparative phytochemical, nutrient and anti-nutrient of stems of Ipomoea Involucrata Beauv, Ipomoea. Triloba L. and Ipomoea Batatas Lam”, Am. J. Food Nutr., 2: 71-76.
  34. Okudaira, R., Kyanba, H., Ichiba, T., Toyokawa, T., “I. Ipomoea extracts with disaccharidase-inhibiting activities”, Jpn. J. Sanit Zool., 17 (2005) 213–221.
  35. Ogunka-Nnoka, C. U., Okolo, L. U., Uwakwe, A. A., “Ameliorative potentials of aqueous extracts of leaf and stem of Ipomoea involucrata on selected bio-chemicals in experimental diabetic rats”, Int. J. Basic Clin. Pharmacol., 9 (2020) 8–15.
  36. Prabhakar, Y., Kamalakar, P., “Green synthesis and characterization of silver nanoparticles from Ipomoea laxiflora: A comprehensive evaluation of antimicrobial efficacy”, Int. J. Herb. Med., 12 (2024) 91-106.
  37. Handayani, W., Yasman, Umar, A., Yudasari, N., “Phytotoxicity study of silver nanoparticles on the germination of water spinach (Ipomoea aquatica Forsk.) and mustard green (Brassica rapa L.) seeds”, IOP Conf. Ser.: Earth Environ. Sci., 1201 (2023) 012080.
  38. Nataraj, K., Vanishree, S., Murali, M., Amruthesh, K. N., “Bio-fabrication, characterization and biological properties of zinc oxide nanoparticles (ZnO-NPs) from scarlet morning glory- Ipomoea hederifolia”, Mater. Chem. Phys., 314 (2024) 128873.
  39. Pavani, K. V., K, G., Banerjee, A., Suresh, S., “Phyto-synthesis of silver nanoparticles using extracts of Ipomoea indica flowers”, American Journal of Nanomaterials, 1 (2013) 5-8.
  40. Varadavenkatesan, T., Selvaraj, R., Vinayagam, R., “Dye degradation and antibacterial activity of green synthesized silver nanoparticles using Ipomoea digitata Linn. flower extract”, Int. J. Environ. Sci. Technol., 16 (2019) 2395-2404.
  41. Bharathi, P. V., Ragavendran, C, Murugan, N, Natarajan, D., “Ipomoea batatas (Convolvulaceae)-mediated synthesis of silver nanoparticles for controlling mosquito vectors of Aedes albopictus, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae)”, Artif. Cells Nanomed. Biotechnol., 45 (2017) 1568-1580.
  42. Rohaeti, E., Rakhmawati, A., “Application of silver nanoparticles synthesized by using Ipomoea batatas L. waste to improve antibacterial properties and hydrophobicity of polyester cloths”, Chiang Mai J. Sci., 45 (2018) 2715-2729.
  43. Wibowo, A., Tajalla, G. U. N., Marsudi, M. A., Cooper, G., Asri, L. A. T. W., Liu, F., Ardy, H., Bartolo, P. J. D. S. “Green synthesis of silver nanoparticles using extract of Cilembu sweet potatoes (Ipomoea batatas L var. Rancing) as potential filler for 3D printed electroactive and anti-infection scaffolds”, Molecules, 26 (2021) 2042.
  44. Nataraj, K., Murali, M., Satish, A., Amruthesh, K. N., “Structural characterization and biological properties of novel biosynthesized zinc oxide nanoparticles from the giant potato plant Ipomoea mauritiana Jacq.”, Biomass Conv. Bioref., 15 (2025) 399-408.
  45. Dudhane, A. A., Waghmode, S. R., Dama, L. B., Mhaindarkar, V. P., Sonawane, A., Katariya, S., “Synthesis and characterization of gold nanoparticles using plant extract of Terminalia arjuna with antibacterial activity”, Int. J. Nanosci. Nanotechnol., 15 (2) (2019) 75-82.
  46. Chandrasekhar, N., Vinay, S. P., “Yellow colored blooms of Argemone mexicana and Turnera ulmifolia mediated synthesis of silver nanoparticles and study of their antibacterial and antioxidant activity”, Appl. Nanosci., 7 (2017) 851–861.
  47. Souri, M., Hoseinpour, V., Ghaemi, N., Shakeri, A., “Procedure optimization for green synthesis of manganese dioxide nanoparticles by Yucca gloriosa leaf extract”, Int. Nano. Lett., 9 (2019) 73–81.
  48. Tesfaye, M., Gonfa, Y., Tadesse, G., Temesgen, T., Periyasamy S., Green synthesis of silver nanoparticles using Vernonia amygdalina plant extract and its antimicrobial activities. Heliyon, 9 (2023) e17356.
  49. Silina, E. V., Ivanova, O. S., Manturova, N. E., Medvedeva, O. A., Shevchenko, A. V., Vorsina, E. S, Achar, R. R., Parfenov, V. A., Stupin, V. A., “Antimicrobial activity of citrate-coated Cerium Oxide nanoparticles”, Nanomaterials (Basel), 14 (2024) 354

 

International Journal of Nanoscience and Nanotechnology
Volume 20, Issue 4
2024
Pages 261-274