International Journal of Nanoscience and Nanotechnology

International Journal of Nanoscience and Nanotechnology

Green Synthesis of Silver Nanoparticle Using Okoubaka Aubrevillei for Antimicrobial Treatment

Document Type : Research Paper

Authors
Department of Chemistry, School of Physical Sciences, Federal University of Technology Owerri, PMB 1526, Imo State, Nigeria
Abstract
   This study prepared silver nanoparticles via the green approach using novel seed pulps of Okoubaka aubrevillei, a medicinal herb. The synthesized silver nanoparticles (AgNPs) were characterized by SEM-EDX, TEM, FTIR, UV-VIS and antimicrobial properties. The surface morphology showed an amorphous and polydispersed spherical shape in a random arrangement in the range of  10 – 50 nm. The nanoparticle shapes observed by TEM were spherical in shape with narrow particle size distribution. The FTIR showed that the extract contains carboxyl (-C=O), hydroxyl (-OH) and amine (N-H) groups which aid in the reduction of Ag+ to Ag0 nanoparticles. The extracts contained a carbonyl group of amino acids which acts as a stabilizing agent to prevent agglomeration.  UV-visible spectra of the AgNPs synthesized by methanolic extract indicated that AgNPs surface Plasmon band occurred in the range of 360 – 380 nm in an aqueous medium and the strongest absorbance bands at 370 nm.  The antimicrobial study showed that the fungi; C. albicans was more susceptible than the bacteria; E. coli, S. aerus, and Ps. aeruginosa, while Ps. aeruginosa is a less susceptible microbe. The synthesized AgNPs were shown to be very effective against C. albicans, and recommended for further application.
Keywords

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