International Journal of Nanoscience and Nanotechnology

International Journal of Nanoscience and Nanotechnology

Preparation of Composites of Antibacterial ‎Materials Based on Bacterial Cellulose ‎and Silver Nanoparticles for Wound ‎Healing

Document Type : Research Paper

Authors
B. Rakhimova 1, 2
K. Kudaibergenov 1
L. Sassykova 1
G. Spanova 1
S. Aknazarov 1, 2
M. Tulepov 1
1 ‎Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, ‎‎050040, Almaty, Kazakhstan
2 Scientific and Production Technical Center “Zhalyn” LLP, 050012, Almaty, Kazakhstan
Abstract
   This study examined the preparation of composites of Bacterial Cellulose (BC) / silver nanoparticles (Ag NPs) using a substrate for the fermentation of the bacteria Gluconacetobacter xylinus C-3 and a reducing agent for the synthesis of silver nanoparticles in situ. The presence of silver in the BC/ Ag NPs composites was confirmed by various characterization tests. Thus, according to Scanning Electron Microscopy, the average size of silver was recorded at 700 nm. X-ray phase analysis showed an increase in the size of cellulose crystallites up to 122 Å with a decrease in the amount of silver nanoparticles. According to UV-visible spectroscopy, an identification peak was found between 420-580 nm. Measurements of the percentage of water absorption and the edge angles of wetting with water confirmed the structures of the resulting nanocomposites - with a smaller pore diameter and a narrow pore distribution over the film size, depending on the silver content. Using the disk diffusion method, excellent antimicrobial activity was shown with a decrease in the number of bacteria in relation to the pathogenic microorganism Staphylococcus (S.) aureus, Pseudomonas (P.) aeruginosa, Escherichia (E.) coli. 
Keywords
Antimicrobial activity
Bacterial cellulose
Composites
Hydrothermal synthesis
Silver nanoparticles
‎Turkevich citrate method.‎

Subjects

  1. Beekmann, U., Zahel, P., Karl, B., Schmölz, L., Börner, F., Gerstmeier, J., Werz, O., Lorkowski, S., Wiegand, C., Fischer, D., Kralisch, D., “Modified bacterial cellulose dressings to treat inflammatory wounds”, Nanomaterials, 10 (2020) 2508.
  2. Amorim, J. D. P., Souza, K. C., Duarte, C. R., Duarte, I. S., Ribeiro, F. A. S., Silva, G. S., Farias, P. M. A., Stingl, A., Costa, A. F. S., Vinhas, G. M., Sarubbo, L. A., “Plant and bacterial nanocellulose: production, properties and applications in medicine, food, cosmetics, electronics and engineering. A review”, Environ Chem Lett, 18 (2020) 851–869.
  3. Moon, R. J., Martini, A., Nairn, J., Simonsen, J., Youngblood, J., “Cellulose nanomaterials review: structure, properties and nanocomposites”, Soc. Rev., 40 (2011) 3941-3994.
  4. Ambekar, R. S., Kandasubramanian, B., “Advancements in nanofibers for wound dressing: A review”, Polym. J., 117 (2019) 304-336.
  5. Felgueiras, H. P., Teixeira, M. A., Tavares, T. D., Homem, N. C., Zille, A., Amorim, M.T.P., “Antimicrobial action and clotting time of thin, hydrated poly(vinyl alcohol)/cellulose acetate films functionalized with LL37 for prospective wound‐healing applications”, Appl. Polym. Sci., 137 (2020) 48626.
  6. Suarato, G., Bertorelli, R., Athanassiou, A. “Borrowing from nature: biopolymers and biocomposites as smart wound care materials”, Bioeng. Biotechnol., 6 (2018), DOI:10.3389/fbioe.2018.00137.
  7. Buruaga-Ramiro, C., Valenzuela, S. V., Valls, C., Roncero, M. B., Pastor, F.I. J., Díaz, P., Martinez, J., “Development of an antimicrobial bioactive paper made from bacterial cellulose”, J. Biol. Macromol., 158 (2020) 587-594.
  8. Zheng, L., Li, Sh., Luo, J., Wang, X., “Latest advances on bacterial cellulose-based antibacterial materials as wound dressings”, Bioeng. Biotechnol., 8 (2020), DOI: 10.3389/fbioe.2020.593768.
  9. Rakhimova, B. U., Kudaibergenov, K. K., Tulepov, M. I., Sassykova, L. R., Spanova, G. A., Mansurov, Z. A., Savitskaya, I. S., “Preparation and determination of the physical and chemical properties of the nano-gel film of bacterial cellulose”, J. Nanomater. Biostructures, 16 (2021) 1587-1593.
  10. Suryanto, H., Muhajir, M., Susilo, B. D., Pradana, Y. R. A., Wijaya, H. W., Ansari, A. S., Yanuhar, U. “Nanofibrillation of bacterial cellulose using high-pressure homogenization and its films characteristics”, Renew. Mater., 9 (2021) 1717-1728.
  11. Pogorelova, N., Rogachev, E., Digel, I., Chernigova, S., Nardin, D. “Bacterial cellulose nanocomposites: morphology and mechanical properties”, Materials, 13 (2020) 2849–2816.
  12. Mirmohammadsadegh, N., Shakoori, M., Moghaddam, H.N., Farhadi, R., Shahverdi, A. R., Amin, M. “Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil”, DARU J. Pharm Sci., (2021), DOI:10.1007/s40199-021-00405-9.
  13. Tabaii, M. J., Emtiazi, G., “Transparent nontoxic antibacterial wound dressing based on silver nano particle/bacterial cellulose nano composite synthesized in the presence of tripolyphosphate”, drug del. sci. tech., 44 (2018) 244-253.
  14. Sukhorukova, I. V., Sheveyko, A. N., Shvindina, N. V., Denisenko, E. A., Ignatov, S. G., Shtansky, D. V., “Approaches for controlled Ag+ ion release: influence of surface topography, roughness, and bactericide content”, ACS Appl. Mater. Interfaces, 9 (2017) 4259-4271.
  15. Paseban, N., Ghadam, P., Purohosseini, P. S., “The fluorscence behavior and stability of AgNP synthesized by juglan regia green husk aqueou extract”, J. Nanosci. Nanotechnol, 15 (2019) 117-126.
  16. John, T., Parmar, K. A., Kotval, Sh. C., Jadhav, J., “Synthesis, characterization, antibacterial ‎and anticancer properties of silver ‎nanoparticles synthesized from carica ‎papaya peel extract”, J. Nanosci. Nanotechnol, 17 (2021) 23-32.
  17. Mittal, D., Narang, K., Leekha, A., Kumar, K., Verma, A.R., “Elucidation of biological activity of silver based nanoparticles using plant constituents of syzygium cumini”, J. Nanosci. Nanotechnol., 15 (2019) 189-198.
  18. Wu, Ch. N., Fuh, Sh. Ch., Lin, Sh. P., Lin, Y. Y., Chen, H. Y., Liu, J. M., Cheng, K. Ch., “TEMPO-oxidized bacterial cellulose pellicle with silver nanoparticles for wound dressing”, Biomacromolecules, 19 (2018) 544-554.
  19. Pal, S., Nisi, R., Stoppa, M., Licciulli, A., “Silver-functionalized bacterial cellulose as antibacterial membrane for wound-healing applications”, ACS Omega, 2 (2017) 3632-3639.
  20. Sajjad, W., Khan, T., Ul-Islam, M., Khan, R., Hussain, Z., Khalid, A., Wahid, F., “Development of modified montmorillonite-bacterial cellulose nanocomposites as a novel substitute for burn skin and tissue regeneration”, Polym., 206 (2019) 548-556.
  21. Sarkandi, A. F., Montazer, M., Harifi, T., Rad, M. M., “Innovative preparation of bacterial cellulose/silver nanocomposite hydrogels: In situ green synthesis, characterization, and antibacterial properties”, Appl. Polym. Sci., 138 (2021) 49824.
  22. Caschera, D., Toro, R. G., Federici, F., Montanari, R., Caro, T., Al-Shemy, M. T., Adel, A. M., “Green approach for the fabrication of silver-oxidized cellulose nanocomposite with antibacterial properties”, Cellulose, 27 (2020) 8059-8073.
  23. Elayaraja, S., Liu, G., Zagorsek, K., Mabrok, M., Ji, M., Ye, Zh., Zhu, S., Rodkhum, Ch., “TEMPO-oxidized biodegradable bacterial cellulose (BBC) membrane coated with biologically-synthesized silver nanoparticles (AgNPs) as a potential antimicrobial agent in aquaculture (In vitro)”, Aquaculture, 530 (2021) 735746.
  24. Spagnol, C., Fragal, E. H., Pereira, A. G.B., Nakamura, C. V., Muniz, E. C., Follmann, H. D. M., Silva, R., Rubira, A. F., “Cellulose nanowhiskers decorated with silver nanoparticles as an additive to antibacterial polymers membranes fabricated by electrospinning”, Colloid Interface Sci., 531 (2018) 705-715.
  25. Lee, K. M., Ngo, Ch. V., Jeong, J. Y., Jeon, E., J. Je, T., Chun, D. M., “Fabrication of an anisotropic superhydrophobic polymer surface using compression molding and dip coating”, Coatings, 7 (2017) 194.
  26. Khatoon, U. T., Nageswara Rao, G. V. S., Mohan, M. K., Ramanaviciene, A., Ramanavicius, A., “Comparative study of antifungal activity of silver and gold nanoparticles synthesized by facile chemical approach”, Environ. Chem. Eng., 6 (2018) 5837-5844.
  27. Garza-Cervantes, J.A., Mendiola-Garza, G., de Melo, E.M., Dugmore, T. I. J., Matharu, A.S., Morones-Ramirez, J.R., “Antimicrobial activity of a silver-microfibrillated cellulose biocomposite against susceptible and resistant bacteria”, Rep., 10 (2020), 7281, DOI:10.1038/s41598-020-64127-9.
  28. Mousavi, S. M., Hashemi, S. A., Ghasemi, Y., Atapour, A., Amani, A.M., Dashtaki, A. S., Babapoor, A., Arjmand, O., “Green synthesis of silver nanoparticles toward bio and medical applications: review study”, Artif Cells Nanomed Biotechnol., 46 (2018) S855-S872.
International Journal of Nanoscience and Nanotechnology
Volume 18, Issue 2
Spring 2022
Pages 123-133